Product nameAnti-TRIM5 delta antibody
DescriptionRabbit polyclonal to TRIM5 delta
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5 delta
- Mouse brain cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab24181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 56 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionCapsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe B box-type zinc finger domain and the coiled-coil domain contribute to the higher and low order multimerization respectively which is essential for restriction activity (PubMed:22482711).
The B30.2/SPRY domain acts as a capsid recognition domain. Polymorphisms in this domain explain the observed species-specific differences among orthologs (PubMed:22482711).
The RING-type zinc finger domain confers E3 ubiquitin ligase activity and is essential for retrovirus restriction activity, autoubiquitination and higher-order multimerization (PubMed:22482711).
modificationsDegraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus.
Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation.
Cellular localizationCytoplasm > P-body. Closely associates with proteasomal subunits in cytoplasmic bodies (By similarity). Colocalizes with SQSTM1 in cytoplasmic bodies.
- Information by UniProt
- EG667823 antibody
- Gm8833 antibody
- RING finger protein 88 antibody
Immunofluorescence of TRIM5 delta in mouse brain tissue with ab24181 at 10 ug/mL.
Anti-TRIM5 delta antibody (ab24181) at 2 µg/ml + Mouse brain lysate
Predicted band size: 56 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
TRIM5 delta is a splice variant of TRIM5 thus the predicted MW of the splice variant differs to the predicted MW of TRIM5.
ab24181 at 1µg/ml staining TRIM delta in mouse brain tissue by IHC
ab24181 has not yet been referenced specifically in any publications.