• Product name

    Anti-TRIM5 delta antibody
  • Description

    Rabbit polyclonal to TRIM5 delta
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5 delta

  • Positive control

    • Mouse brain cell lysate.



Our Abpromise guarantee covers the use of ab24181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 1 µg/ml.


  • Function

    Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The B box-type zinc finger domain and the coiled-coil domain contribute to the higher and low order multimerization respectively which is essential for restriction activity (PubMed:22482711).
    The B30.2/SPRY domain acts as a capsid recognition domain. Polymorphisms in this domain explain the observed species-specific differences among orthologs (PubMed:22482711).
    The RING-type zinc finger domain confers E3 ubiquitin ligase activity and is essential for retrovirus restriction activity, autoubiquitination and higher-order multimerization (PubMed:22482711).
  • Post-translational

    Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus.
    Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation.
  • Cellular localization

    Cytoplasm > P-body. Closely associates with proteasomal subunits in cytoplasmic bodies (By similarity). Colocalizes with SQSTM1 in cytoplasmic bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • EG667823 antibody
    • Gm8833 antibody
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • Tripartite motif containing 5 transcript variant iota antibody
    • Tripartite motif containing 5 transcript variant kappa antibody
    • Tripartite motif containing protein 5 antibody
    • Tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all


  • Anti-TRIM5 delta antibody (ab24181) at 2 µg/ml + Mouse brain lysate

    Predicted band size: 56 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

    TRIM5 delta is a splice variant of TRIM5 thus the predicted MW of the splice variant differs to the predicted MW of TRIM5.
  • Immunofluorescence of TRIM5 delta in mouse brain tissue with ab24181 at 10 ug/mL.

  • ab24181 at 1µg/ml staining TRIM delta in mouse brain tissue by IHC


ab24181 has not yet been referenced specifically in any publications.

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