Key features and details
- Rabbit polyclonal to TRIM5 gamma
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM5 gamma antibody
DescriptionRabbit polyclonal to TRIM5 gamma
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (Human) (C terminal).
- Human bladder and colon cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa.|
FunctionIsoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
modificationsUbiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
Cellular localizationCytoplasm > P-body. Cytoplasmic bodies.
- Information by UniProt
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
All lanes : Anti-TRIM5 gamma antibody (ab17203) at 2 µg/ml
Lane 1 : Human bladder cell lysate
Lane 2 : Human colon cell lysate
Observed band size: 35 kDa why is the actual band size different from the predicted?
Detection of TRIM5 gamma in human bladder (A) and colon (B) cell lysates. ab17203 used at 2 ug/ml. Band of ~35 kDa detected.
Immunofluorescence of TRIM5 gamma in Human bladder tissue with ab17203 at 20 ug/mL.
ab17203 at 2µg/ml staining TRIM5 gamma in human bladder tissue by IHC
ab17203 has not yet been referenced specifically in any publications.