Synthetic peptide within Human TRIM50 aa 361-391 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q86XT4
Our Abpromise guarantee covers the use of ab174880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 55 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling TRIM50 with ab174880 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells labeling TRIM50 with ab174880 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"