Overview

  • Product name
    Anti-TRIM50 antibody - C-terminal
    See all TRIM50 primary antibodies
  • Description
    Rabbit polyclonal to TRIM50 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRIM50 aa 361-391 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q86XT4

  • Positive control
    • Jurkat cells and cell line lysates; Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174880 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 55 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    E3 ubiquitin-protein ligase.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin ligase antibody
    • E3 ubiquitin protein ligase TRIM50 antibody
    • E3 ubiquitin-protein ligase TRIM50 antibody
    • TRI50_HUMAN antibody
    • TRIM50 antibody
    • TRIM50A antibody
    • Tripartite motif containing 50 antibody
    • Tripartite motif containing protein 50 antibody
    • Tripartite motif protein 50 antibody
    • tripartite motif protein 50A antibody
    • tripartite motif-containing 50A antibody
    • Tripartite motif-containing protein 50 antibody
    see all

Images

  • Anti-TRIM50 antibody - C-terminal (ab174880) at 1/100 dilution + Jurkat cell line lysates at 15 µg

    Predicted band size: 55 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling TRIM50 with ab174880 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of Jurkat cells labeling TRIM50 with ab174880 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Ma X  et al. TRIM50 suppressed hepatocarcinoma progression through directly targeting SNAIL for ubiquitous degradation. Cell Death Dis 9:608 (2018). Read more (PubMed: 29789583) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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