• Product name
    Anti-TRIM56 antibody [EPR10583]
    See all TRIM56 primary antibodies
  • Description
    Rabbit monoclonal [EPR10583] to TRIM56
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues within Human TRIM56 (Uniprot ID Q9BRZ2).

  • Positive control
    • HeLa, A375, MCF7 cell lysate; Human brain tissue; Human pancreas tissue; MCF7 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab154862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 81 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked polyubiquitination of TMEM173/STING, thereby playing a key role in innate immunity. TMEM173/STING 'Lys-63'-linked ubiquitination activates the production of type I interferon IFN-beta following detection of pathogen- and host-derived double-stranded DNA.
    • Sequence similarities
      Belongs to the TRIM/RBCC family.
      Contains 2 B box-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • A130009K11Rik antibody
      • DKFZp667O116 antibody
      • E3 ubiquitin-protein ligase TRIM56 antibody
      • FLJ35608 antibody
      • Gm452 antibody
      • MGC37358 antibody
      • OTTMUSP00000027392 antibody
      • RING finger protein 109 antibody
      • RNF109 antibody
      • TRI56_HUMAN antibody
      • Trim56 antibody
      • Tripartite motif containing protein 56 antibody
      • Tripartite motif-containing protein 56 antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TRIM56 knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: A375 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab154862 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab154862 was shown to recognize TRIM56 when TRIM56 knockout samples were used, along with additional cross-reactive bands. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154862 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-TRIM56 antibody [EPR10583] (ab154862) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 81 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TRIM56 with ab154862 at 1/50.
    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TRIM56 with ab154862 at 1/50.
    • Immunofluorescent analysis of MCF7 cells labeling TRIM56 with ab154862 at 1/100.


    This product has been referenced in:
    • Fiskin E  et al. Structural basis for the recognition and degradation of host TRIM proteins by Salmonella effector SopA. Nat Commun 8:14004 (2017). WB . Read more (PubMed: 28084320) »
    See 1 Publication for this product

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