Key features and details
- Rabbit polyclonal to TRIM58
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM58 antibody
DescriptionRabbit polyclonal to TRIM58
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: PC3 cell lysate. IHC-P: Human bone marrow, prostate and placenta and testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 55 kDa.|
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- BIA2 antibody
- DKFZp434C091 antibody
- Protein BIA2 antibody
All lanes : Anti-TRIM58 antibody (ab254768) at 0.4 µg/ml
Lane 1 : RT4 cell lysate
Lane 2 : CACO-2 cell lysate
Predicted band size: 55 kDa
Paraffin-embedded human testis tissue stained for TRIM58 using ab254768 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for TRIM58 using ab254768 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human bone marrow tissue stained for TRIM58 using ab254768 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for TRIM58 using ab254768 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for TRIM58 using ab254768 at 1/200 dilution in immunohistochemical analysis.
ab254768 has not yet been referenced specifically in any publications.