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    trim62-antibody-ab154635.pdf

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Anti-TRIM62 antibody (ab154635)

  • Datasheet
  • SDS
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Western blot - Anti-TRIM62 antibody (ab154635)
  • Western blot - Anti-TRIM62 antibody (ab154635)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)

Key features and details

  • Rabbit polyclonal to TRIM62
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-TRIM62 antibody
    See all TRIM62 primary antibodies
  • Description

    Rabbit polyclonal to TRIM62
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 116-453 of Human TRIM62 (UniProt ID: Q9BVG3).

  • Positive control

    • A431 whole cell lysate. A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Recombinant fragment
All applications
Mouse
Cow
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING finger is required for ubiquitin ligase activity.
  • Post-translational
    modifications

    Polyubiquitinated, autoubiquitinated in the presence of UBE2D2.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9BVG3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55223 Human
    • Entrez Gene: 67525 Mouse
    • SwissProt: Q9BVG3 Human
    • SwissProt: Q80V85 Mouse
    • Unigene: 656006 Human
    • Unigene: 30653 Mouse
    • Alternative names

      • 6330414G21Rik antibody
      • AI450348 antibody
      • DEAR1 antibody
      • ductal epithelium-associated RING Chromosome 1 antibody
      • E3 ubiquitin-protein ligase TRIM62 antibody
      • FLJ10759 antibody
      • FLJ16558 antibody
      • MGC115263 antibody
      • TRI62_HUMAN antibody
      • TRIM62 antibody
      • Tripartite motif containing 62 antibody
      • Tripartite motif-containing protein 62 antibody
      see all

    Images

    • Western blot - Anti-TRIM62 antibody (ab154635)
      Western blot - Anti-TRIM62 antibody (ab154635)
      Anti-TRIM62 antibody (ab154635) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 54 kDa



      10% SDS PAGE
    • Western blot - Anti-TRIM62 antibody (ab154635)
      Western blot - Anti-TRIM62 antibody (ab154635)
      Anti-TRIM62 antibody (ab154635) at 1/90000 dilution + 100ng of 37kDa recombinant TRIM62 protein antigen.

      Predicted band size: 54 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)
      Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)
      Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling TRIM62 with ab154635 at a 1/200 dilution.
      The lower image has been stained with Hoechst 44432.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab154635? Please let us know so that we can cite the reference in this datasheet.

    ab154635 has been referenced in 1 publication.

    • Lin XF  et al. Therapeutic effect of psoralen on muscle atrophy induced by tumor necrosis factor-a. Iran J Basic Med Sci 23:251-256 (2020). PubMed: 32405369

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