Anti-TRIM62 antibody (ab154635)
Key features and details
- Rabbit polyclonal to TRIM62
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-TRIM62 antibody
See all TRIM62 primary antibodies -
Description
Rabbit polyclonal to TRIM62 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 116-453 of Human TRIM62 (UniProt ID: Q9BVG3).
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Positive control
- A431 whole cell lysate. A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
Recombinant fragment
|
| All applications |
Mouse
Cow
Xenopus tropicalis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
Domain
The RING finger is required for ubiquitin ligase activity. -
Post-translational
modificationsPolyubiquitinated, autoubiquitinated in the presence of UBE2D2. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55223 Human
- Entrez Gene: 67525 Mouse
- SwissProt: Q9BVG3 Human
- SwissProt: Q80V85 Mouse
- Unigene: 656006 Human
- Unigene: 30653 Mouse
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Alternative names
- 6330414G21Rik antibody
- AI450348 antibody
- DEAR1 antibody
see all
Images
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Western blot - Anti-TRIM62 antibody (ab154635)Anti-TRIM62 antibody (ab154635) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Western blot - Anti-TRIM62 antibody (ab154635)Anti-TRIM62 antibody (ab154635) at 1/90000 dilution + 100ng of 37kDa recombinant TRIM62 protein antigen.
Predicted band size: 54 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling TRIM62 with ab154635 at a 1/200 dilution.
The lower image has been stained with Hoechst 44432.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154635 has been referenced in 1 publication.
- Lin XF et al. Therapeutic effect of psoralen on muscle atrophy induced by tumor necrosis factor-a. Iran J Basic Med Sci 23:251-256 (2020). PubMed: 32405369