Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM62
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 116-453 of Human TRIM62 (UniProt ID: Q9BVG3).

  • Positive control
    • A431 whole cell lysate. A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING finger is required for ubiquitin ligase activity.
  • Post-translational
    modifications
    Polyubiquitinated, autoubiquitinated in the presence of UBE2D2.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6330414G21Rik antibody
    • AI450348 antibody
    • DEAR1 antibody
    • ductal epithelium-associated RING Chromosome 1 antibody
    • E3 ubiquitin-protein ligase TRIM62 antibody
    • FLJ10759 antibody
    • FLJ16558 antibody
    • MGC115263 antibody
    • TRI62_HUMAN antibody
    • TRIM62 antibody
    • Tripartite motif containing 62 antibody
    • Tripartite motif-containing protein 62 antibody
    see all

Images

  • Anti-TRIM62 antibody (ab154635) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Anti-TRIM62 antibody (ab154635) at 1/90000 dilution + 100ng of 37kDa recombinant TRIM62 protein antigen.

    Predicted band size: 54 kDa

  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling TRIM62 with ab154635 at a 1/200 dilution.
    The lower image has been stained with Hoechst 44432.

References

ab154635 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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