Overview

  • Product name
    Anti-TRIM62 antibody
    See all TRIM62 primary antibodies
  • Description
    Rabbit polyclonal to TRIM62
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 116-453 of Human TRIM62 (UniProt ID: Q9BVG3).

  • Positive control
    • A431 whole cell lysate. A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING finger is required for ubiquitin ligase activity.
  • Post-translational
    modifications
    Polyubiquitinated, autoubiquitinated in the presence of UBE2D2.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • 6330414G21Rik antibody
      • AI450348 antibody
      • DEAR1 antibody
      • ductal epithelium-associated RING Chromosome 1 antibody
      • E3 ubiquitin-protein ligase TRIM62 antibody
      • FLJ10759 antibody
      • FLJ16558 antibody
      • MGC115263 antibody
      • TRI62_HUMAN antibody
      • TRIM62 antibody
      • Tripartite motif containing 62 antibody
      • Tripartite motif-containing protein 62 antibody
      see all

    Images

    • Western blot - Anti-TRIM62 antibody (ab154635)
      Western blot - Anti-TRIM62 antibody (ab154635)
      Anti-TRIM62 antibody (ab154635) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 54 kDa



      10% SDS PAGE
    • Western blot - Anti-TRIM62 antibody (ab154635)
      Western blot - Anti-TRIM62 antibody (ab154635)
      Anti-TRIM62 antibody (ab154635) at 1/90000 dilution + 100ng of 37kDa recombinant TRIM62 protein antigen.

      Predicted band size: 54 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)
      Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)
      Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling TRIM62 with ab154635 at a 1/200 dilution.
      The lower image has been stained with Hoechst 44432.

    References

    ab154635 has not yet been referenced specifically in any publications.

    Publishing research using ab154635? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab154635.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up