Anti-TRIM62 antibody (ab154635)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-TRIM62 antibody
See all TRIM62 primary antibodies -
DescriptionRabbit polyclonal to TRIM62
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 116-453 of Human TRIM62 (UniProt ID: Q9BVG3).
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Positive control
- A431 whole cell lysate. A431 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 54 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionE3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
DomainThe RING finger is required for ubiquitin ligase activity.
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Post-translational
modificationsPolyubiquitinated, autoubiquitinated in the presence of UBE2D2. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 55223 Human
- Entrez Gene: 67525 Mouse
- SwissProt: Q9BVG3 Human
- SwissProt: Q80V85 Mouse
- Unigene: 656006 Human
- Unigene: 30653 Mouse
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Alternative names
- 6330414G21Rik antibody
- AI450348 antibody
- DEAR1 antibody
see all
Images
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Western blot - Anti-TRIM62 antibody (ab154635)Anti-TRIM62 antibody (ab154635) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Western blot - Anti-TRIM62 antibody (ab154635)Anti-TRIM62 antibody (ab154635) at 1/90000 dilution + 100ng of 37kDa recombinant TRIM62 protein antigen.
Predicted band size: 54 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling TRIM62 with ab154635 at a 1/200 dilution.
The lower image has been stained with Hoechst 44432.
References
ab154635 has not yet been referenced specifically in any publications.
