Anti-TRIM62 antibody (ab154635)

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Overview

  • Product name

    Anti-TRIM62 antibody
    See all TRIM62 primary antibodies

  • Description

    Rabbit polyclonal to TRIM62

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus tropicalis

  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 116-453 of Human TRIM62 (UniProt ID: Q9BVG3).

  • Positive control

    • A431 whole cell lysate. A431 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.

  • Domain

    The RING finger is required for ubiquitin ligase activity.

  • Post-translational
    modifications

    Polyubiquitinated, autoubiquitinated in the presence of UBE2D2.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • 6330414G21Rik antibody
      • AI450348 antibody
      • DEAR1 antibody
      • ductal epithelium-associated RING Chromosome 1 antibody
      • E3 ubiquitin-protein ligase TRIM62 antibody
      • FLJ10759 antibody
      • FLJ16558 antibody
      • MGC115263 antibody
      • TRI62_HUMAN antibody
      • TRIM62 antibody
      • Tripartite motif containing 62 antibody
      • Tripartite motif-containing protein 62 antibody
      see all

    Images

    • Western blot - Anti-TRIM62 antibody (ab154635)
      Western blot - Anti-TRIM62 antibody (ab154635)
      Anti-TRIM62 antibody (ab154635) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 54 kDa



      10% SDS PAGE
    • Western blot - Anti-TRIM62 antibody (ab154635)
      Western blot - Anti-TRIM62 antibody (ab154635)
      Anti-TRIM62 antibody (ab154635) at 1/90000 dilution + 100ng of 37kDa recombinant TRIM62 protein antigen.

      Predicted band size: 54 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)
      Immunocytochemistry/ Immunofluorescence - Anti-TRIM62 antibody (ab154635)
      Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling TRIM62 with ab154635 at a 1/200 dilution.
      The lower image has been stained with Hoechst 44432.

    References

    ab154635 has not yet been referenced specifically in any publications.

    Publishing research using ab154635? Please let us know so that we can cite the reference in this datasheet.

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