Product nameAnti-TRIM62 antibody
See all TRIM62 primary antibodies
DescriptionRabbit polyclonal to TRIM62
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- Jurkat cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab51039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionE3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe RING finger is required for ubiquitin ligase activity.
modificationsPolyubiquitinated, autoubiquitinated in the presence of UBE2D2.
- Information by UniProt
- 6330414G21Rik antibody
- AI450348 antibody
- DEAR1 antibody
Anti-TRIM62 antibody (ab51039) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
ab51039 has not yet been referenced specifically in any publications.