Anti-TRIM71/LIN41 antibody (ab105330)
Key features and details
- Rabbit polyclonal to TRIM71/LIN41
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM71/LIN41 antibody -
Description
Rabbit polyclonal to TRIM71/LIN41 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TRIM71/LIN41 (C terminal).
Database link: Q2Q1W2 -
Positive control
- Human brain tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
|
ICC |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa. |
ICC
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1. -
Tissue specificity
Specifically expressed in testis. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsAutoubiquitinated. -
Cellular localization
Cytoplasm, P-body. - Information by UniProt
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Database links
- Entrez Gene: 131405 Human
- SwissProt: Q2Q1W2 Human
- Unigene: 113170 Human
- Unigene: 567678 Human
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Alternative names
- 2610206G21Rik antibody
- Abnormal cell LINeage LIN 41 antibody
- AL022943 antibody
see all
Images
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Lane 1 : Anti-TRIM71/LIN41 antibody (ab105330) at 1 µg/ml
Lane 2 : Anti-TRIM71/LIN41 antibody (ab105330) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-rabbit secondary antibody
Predicted band size: 93 kDa -
Immunohistochemistry of TRIM71/LIN41 in human brain tissue with ab105330 at 5 µg/ml.
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Immunofluorescence of TRIM71/LIN41 in Human Brain cells using ab105330 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab105330 has been referenced in 2 publications.
- Han F et al. MicroRNA-4491 enhances cell proliferation and inhibits cell apoptosis in non-small cell lung cancer via targeting TRIM7. Oncol Lett 22:591 (2021). PubMed: 34149902
- Robinton DA et al. The Lin28/let-7 Pathway Regulates the Mammalian Caudal Body Axis Elongation Program. Dev Cell 48:396-405.e3 (2019). PubMed: 30661985