Anti-TRIM71/LIN41 antibody (ab105330)
Key features and details
- Rabbit polyclonal to TRIM71/LIN41
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM71/LIN41 antibody
See all TRIM71/LIN41 primary antibodies -
Description
Rabbit polyclonal to TRIM71/LIN41 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human TRIM71/LIN41 (C terminal).
Database link: NP_001034200 -
Positive control
- Human brain tissue lysate
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General notes
This product was previously labelled as TRIM71
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab105330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa. | |
| ICC | Use a concentration of 5 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1. -
Tissue specificity
Specifically expressed in testis. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsAutoubiquitinated. -
Cellular localization
Cytoplasm, P-body. - Information by UniProt
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Database links
- Entrez Gene: 131405 Human
- Entrez Gene: 636931 Mouse
- Entrez Gene: 301042 Rat
- SwissProt: Q2Q1W2 Human
- SwissProt: Q1PSW8 Mouse
- SwissProt: D3ZVM4 Rat
- Unigene: 113170 Human
- Unigene: 567678 Human
see all -
Alternative names
- 2610206G21Rik antibody
- Abnormal cell LINeage LIN 41 antibody
- AL022943 antibody
see all
Images
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Western blot - Anti-TRIM71/LIN41 antibody (ab105330)Lane 1 : Anti-TRIM71/LIN41 antibody (ab105330) at 1 µg/ml
Lane 2 : Anti-TRIM71/LIN41 antibody (ab105330) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-rabbit secondary antibody
Predicted band size: 93 kDa -
Immunocytochemistry - Anti-TRIM71/LIN41 antibody (ab105330)Immunohistochemistry of TRIM71/LIN41 in human brain tissue with ab105330 at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-TRIM71/LIN41 antibody (ab105330)Immunofluorescence of TRIM71/LIN41 in Human Brain cells using ab105330 at 20 ug/ml.
Protocols
Datasheets and documents
References (1)
ab105330 has been referenced in 1 publication.
- Robinton DA et al. The Lin28/let-7 Pathway Regulates the Mammalian Caudal Body Axis Elongation Program. Dev Cell 48:396-405.e3 (2019). PubMed: 30661985
