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Anti-TRIM71/LIN41 antibody (ab105330)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-TRIM71/LIN41 antibody (ab105330)
  • Immunocytochemistry - Anti-TRIM71/LIN41 antibody (ab105330)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM71/LIN41 antibody (ab105330)

Key features and details

  • Rabbit polyclonal to TRIM71/LIN41
  • Suitable for: ICC/IF, WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-TRIM71/LIN41 antibody
    See all TRIM71/LIN41 primary antibodies
  • Description

    Rabbit polyclonal to TRIM71/LIN41
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRIM71/LIN41 (C terminal).
    Database link: NP_001034200

  • Positive control

    • Human brain tissue lysate
  • General notes

     This product was previously labelled as TRIM71

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab105330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
ICC Use a concentration of 5 µg/ml.

Target

  • Function

    E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
  • Tissue specificity

    Specifically expressed in testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Autoubiquitinated.
  • Cellular localization

    Cytoplasm, P-body.
  • Target information above from: UniProt accession Q2Q1W2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 131405 Human
    • Entrez Gene: 636931 Mouse
    • Entrez Gene: 301042 Rat
    • SwissProt: Q2Q1W2 Human
    • SwissProt: Q1PSW8 Mouse
    • SwissProt: D3ZVM4 Rat
    • Unigene: 113170 Human
    • Unigene: 567678 Human
    • Unigene: 17857 Mouse
    • Unigene: 225076 Rat
    see all
  • Alternative names

    • 2610206G21Rik antibody
    • Abnormal cell LINeage LIN 41 antibody
    • AL022943 antibody
    • E3 ubiquitin-protein ligase TRIM71 antibody
    • Gm1127 antibody
    • Homolog of C. elegans Lin 41 antibody
    • LIN 41 antibody
    • LIN41 antibody
    • LIN41_HUMAN antibody
    • mlin 41 antibody
    • Protein lin 41 homolog antibody
    • Protein lin-41 homolog antibody
    • RGD1566388 antibody
    • Ripply2 antibody
    • TRIM 71 antibody
    • TRIM71 antibody
    • Tripartite motif containing 71 antibody
    • Tripartite motif-containing protein 71 antibody
    see all

Images

  • Western blot - Anti-TRIM71/LIN41 antibody (ab105330)
    Western blot - Anti-TRIM71/LIN41 antibody (ab105330)
    Lane 1 : Anti-TRIM71/LIN41 antibody (ab105330) at 1 µg/ml
    Lane 2 : Anti-TRIM71/LIN41 antibody (ab105330) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti-rabbit secondary antibody

    Predicted band size: 93 kDa

  • Immunocytochemistry - Anti-TRIM71/LIN41 antibody (ab105330)
    Immunocytochemistry - Anti-TRIM71/LIN41 antibody (ab105330)

    Immunohistochemistry of TRIM71/LIN41 in human brain tissue with ab105330 at 5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM71/LIN41 antibody (ab105330)
    Immunocytochemistry/ Immunofluorescence - Anti-TRIM71/LIN41 antibody (ab105330)

    Immunofluorescence of TRIM71/LIN41 in Human Brain cells using ab105330 at 20 ug/ml.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab105330? Please let us know so that we can cite the reference in this datasheet.

    ab105330 has been referenced in 1 publication.

    • Robinton DA  et al. The Lin28/let-7 Pathway Regulates the Mammalian Caudal Body Axis Elongation Program. Dev Cell 48:396-405.e3 (2019). PubMed: 30661985

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TRIM71 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (human embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    human embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Miguel Fidalgo

    Verified customer

    Submitted Nov 10 2015

    Western blot abreview for Anti-TRIM71 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (HeLa)
    Loading amount
    20 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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