Anti-TRIM71/LIN41 antibody (ab105330)

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Overview

  • Product name

    Anti-TRIM71/LIN41 antibody
    See all TRIM71/LIN41 primary antibodies

  • Description

    Rabbit polyclonal to TRIM71/LIN41

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human TRIM71/LIN41 (C terminal).
    Database link: NP_001034200

  • Positive control

    • Human brain tissue lysate

  • General notes

     This product was previously labelled as TRIM71

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab105330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.
ICC Use a concentration of 5 µg/ml.

Target

  • Function

    E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.

  • Tissue specificity

    Specifically expressed in testis.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.

  • Post-translational
    modifications

    Autoubiquitinated.

  • Cellular localization

    Cytoplasm, P-body.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2610206G21Rik antibody
    • Abnormal cell LINeage LIN 41 antibody
    • AL022943 antibody
    • E3 ubiquitin-protein ligase TRIM71 antibody
    • Gm1127 antibody
    • Homolog of C. elegans Lin 41 antibody
    • LIN 41 antibody
    • LIN41 antibody
    • LIN41_HUMAN antibody
    • mlin 41 antibody
    • Protein lin 41 homolog antibody
    • Protein lin-41 homolog antibody
    • RGD1566388 antibody
    • Ripply2 antibody
    • TRIM 71 antibody
    • TRIM71 antibody
    • Tripartite motif containing 71 antibody
    • Tripartite motif-containing protein 71 antibody
    see all

Images

  • Western blot - Anti-TRIM71/LIN41 antibody (ab105330)
    Western blot - Anti-TRIM71/LIN41 antibody (ab105330)
    Lane 1 : Anti-TRIM71/LIN41 antibody (ab105330) at 1 µg/ml
    Lane 2 : Anti-TRIM71/LIN41 antibody (ab105330) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti-rabbit secondary antibody

    Predicted band size: 93 kDa

  • Immunocytochemistry - Anti-TRIM71/LIN41 antibody (ab105330)
    Immunocytochemistry - Anti-TRIM71/LIN41 antibody (ab105330)

    Immunohistochemistry of TRIM71/LIN41 in human brain tissue with ab105330 at 5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM71/LIN41 antibody (ab105330)
    Immunocytochemistry/ Immunofluorescence - Anti-TRIM71/LIN41 antibody (ab105330)

    Immunofluorescence of TRIM71/LIN41 in Human Brain cells using ab105330 at 20 ug/ml.

References

ab105330 has not yet been referenced specifically in any publications.

Publishing research using ab105330? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-TRIM71 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (human embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
human embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Mr. Miguel Fidalgo

Verified customer

Submitted Nov 10 2015

Western blot abreview for Anti-TRIM71 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (HeLa)
Loading amount
20 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jun 09 2011

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