Anti-TRIO antibody (ab194365)
Key features and details
- Mouse polyclonal to TRIO
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIO antibody
See all TRIO primary antibodies -
Description
Mouse polyclonal to TRIO -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human TRIO aa 1950-2100. NP_009049.
Database link: O75962 -
Positive control
- HeLa cell lysate; recombinant Human TRIO protein (immunogen).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 347 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 347 kDa. |
Target
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Function
Promotes the exchange of GDP by GTP. Together with leukocyte antigen-related (LAR) protein, it could play a role in coordinating cell-matrix and cytoskeletal rearrangements necessary for cell migration and cell growth. -
Tissue specificity
Highly expressed in heart, skeletal muscle, brain, pancreas, placenta, liver, kidney and lung. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 CRAL-TRIO domain.
Contains 2 DH (DBL-homology) domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 PH domains.
Contains 1 protein kinase domain.
Contains 2 SH3 domains.
Contains 4 spectrin repeats. -
Domain
The N-terminal DBL/GEF domain specifically catalyzes nucleotide exchange for RAC1, leading to the activation of Jun kinase and the production of membrane ruffles. The second DBL/GEF domain is an exchange factor for rhoa and induces the formation of stress fibers. -
Post-translational
modificationsPhosphorylated on serine residue(s). -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7204 Human
- Entrez Gene: 223435 Mouse
- Omim: 601893 Human
- SwissProt: O75962 Human
- SwissProt: Q0KL02 Mouse
- Unigene: 130031 Human
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Alternative names
- ARHGEF23 antibody
- MEBAS antibody
- MRD44 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab194365 has been referenced in 2 publications.
- Wang J et al. Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA. Cell Death Dis 12:1148 (2021). PubMed: 34893584
- Paskus JD et al. Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action. Cell Rep 29:2944-2952.e5 (2019). PubMed: 31801062