Product nameTriose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric)
Sample typeSerum, Plasma, Tissue, Adherent cells, Suspension cells
Assay typeEnzyme activity
Sensitivity> 40 mU/ml
Assay time0h 30m
Species reactivityReacts with: Other species, Mammals
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.
TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min
Triose Phosphate Isomerase (TPI, TIM, EC 184.108.40.206) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests NADH Standard Yellow 1 vial Positive Control 1 vial TPI Assay Buffer 1 x 25ml TPI Developer 1 vial TPI Enzyme Mix 1 vial TPI Substrate 1 vial
PathwayCarbohydrate biosynthesis; gluconeogenesis.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Involvement in diseaseTriosephosphate isomerase deficiency
Sequence similaritiesBelongs to the triosephosphate isomerase family.
modificationsThe initiator methionine for isoform 2 is removed.
- Information by UniProt
ab197001 has not yet been referenced specifically in any publications.