Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001)

Overview

  • Product name

    Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Sensitivity

    > 40 mU/ml
  • Assay time

    0h 30m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.


    The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.


    TPI assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with microplate reader every 2-3 min for 20-40 min

  • Notes

    Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.

    In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    NADH Standard Yellow 1 vial
    Positive Control 1 vial
    TPI Assay Buffer 1 x 25ml
    TPI Developer 1 vial
    TPI Enzyme Mix 1 vial
    TPI Substrate 1 vial
  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
  • Involvement in disease

    Triosephosphate isomerase deficiency
  • Sequence similarities

    Belongs to the triosephosphate isomerase family.
  • Post-translational
    modifications

    The initiator methionine for isoform 2 is removed.
  • Information by UniProt
  • Alternative names

    • TIM
    • TPI1
    • TPIS_HUMAN
    • Triose-phosphate isomerase
    • Triosephosphate isomerase
    see all

Images

  • Relative TPI activity was calculated in lysates prepared from rat muscle (0.5 µg), MCF-7 cells (0.8 µg), and HeLa cells (0.84 µg). 

  • Typical NADH Standard Curve obtained following assay protocol.

  • Kinetic measurement of Triose Phosphate Isomerase (TPI) activity in various samples.

Protocols

References

ab197001 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197001.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up