Product nameTriose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric)
Sample typeSerum, Plasma, Tissue, Adherent cells, Suspension cells
Assay typeEnzyme activity
Sensitivity> 40 mU/ml
Assay time0h 30m
Species reactivityReacts with: Other species, Mammals
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.
TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min
Triose Phosphate Isomerase (TPI, TIM, EC 126.96.36.199) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests NADH Standard Yellow 1 vial Positive Control 1 vial TPI Assay Buffer 1 x 25ml TPI Developer 1 vial TPI Enzyme Mix 1 vial TPI Substrate 1 vial
PathwayCarbohydrate biosynthesis; gluconeogenesis.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Involvement in diseaseTriosephosphate isomerase deficiency
Sequence similaritiesBelongs to the triosephosphate isomerase family.
modificationsThe initiator methionine for isoform 2 is removed.
- Information by UniProt
ab197001 has not yet been referenced specifically in any publications.