Key features and details
- Rabbit polyclonal to Triosephosphate isomerase
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Triosephosphate isomerase antibody
See all Triosephosphate isomerase primary antibodies
DescriptionRabbit polyclonal to Triosephosphate isomerase
Specificityab196618 detects endogenous level of total Triosephosphate isomerase protein.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant full length protein within Human Triosephosphate isomerase. The exact sequence is proprietary.
Database link: P60174
- 22RV-1, BT474, HeLa, Jurkat, U251 and HepG2 cell lysates; mouse kidney, brain and heart lysates. Human kidney and mouse lung tissues. HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Jurkat whole cell lysate (ab7899)
Our Abpromise guarantee covers the use of ab196618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 31 kDa.|
RelevanceTriosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
Cellular localizationCytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
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All lanes : Anti-Triosephosphate isomerase antibody (ab196618) at 1/500 dilution
Lane 1 : 22RV-1 cell lysate
Lane 2 : BT474 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : U251 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : mouse kidney lysate
Lane 8 : mouse brain lysate
Lane 9 : mouse heart lysate
Predicted band size: 31 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Triosephosphate isomerase using ab196618 at a 1/200 dilution
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Triosephosphate isomerase using ab196618 at a 1/200 dilution.
Immunofluorescence analysis of HeLa cells, labeling Triosephosphate isomerase using ab196618 at a 1/50 dilution. Blue: DAPI for nuclear staining.
ab196618 has not yet been referenced specifically in any publications.