Overview

  • Product name

    Anti-Triosephosphate isomerase antibody
    See all Triosephosphate isomerase primary antibodies
  • Description

    Rabbit polyclonal to Triosephosphate isomerase
  • Host species

    Rabbit
  • Specificity

    ab196618 detects endogenous level of total Triosephosphate isomerase protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein within Human Triosephosphate isomerase. The exact sequence is proprietary.
    Database link: P60174

  • Positive control

    • 22RV-1, BT474, HeLa, Jurkat, U251 and HepG2 cell lysates; mouse kidney, brain and heart lysates. Human kidney and mouse lung tissues. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 31 kDa.

Target

  • Relevance

    Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
  • Cellular localization

    Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
  • Database links

  • Alternative names

    • epididymis secretory protein Li 49 antibody
    • HEL-S-49 antibody
    • MGC88108 antibody
    • TIM antibody
    • TPI 1 antibody
    • TPI antibody
    • TPI1 antibody
    • TPID antibody
    • Triose phosphate isomerase 1 antibody
    • Triose phosphate isomerase antibody
    • Triosephosphate isomerase 1 antibody
    • Triosephosphate isomerase antibody
    see all

Images

  • All lanes : Anti-Triosephosphate isomerase antibody (ab196618) at 1/500 dilution

    Lane 1 : 22RV-1 cell lysate
    Lane 2 : BT474 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : U251 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : mouse kidney lysate
    Lane 8 : mouse brain lysate
    Lane 9 : mouse heart lysate

    Predicted band size: 31 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Triosephosphate isomerase using ab196618 at a 1/200 dilution

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Triosephosphate isomerase using ab196618 at a 1/200 dilution.

  • Immunofluorescence analysis of HeLa cells, labeling Triosephosphate isomerase using ab196618 at a 1/50 dilution. Blue: DAPI for nuclear staining.

References

ab196618 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196618.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up