Product nameAnti-Triosephosphate isomerase antibody
See all Triosephosphate isomerase primary antibodies
DescriptionGoat polyclonal to Triosephosphate isomerase
SpecificityThis antibody is expected to recognise both reported isoforms.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog
- Human liver lysate, HepG2 whole cell lysate (ab7900).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab28760 in the following tested applications.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceTriosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
Cellular localizationCytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
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Anti-Triosephosphate isomerase antibody (ab28760) at 0.01 µg/ml + human liver lysate 30µg total protein per lane
Predicted band size: 26 kDa
Observed band size: 26 kDa
Primary incubated for 1 hour.
Detected by chemiluminescence.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining Triosephosphate isomerase with ab28760 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a Tris/EDTA buffer (pH 9).
This product has been referenced in:
- Zhang CS et al. Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Nature 548:112-116 (2017). Read more (PubMed: 28723898) »
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »