Overview

  • Product name

    Anti-Triosephosphate isomerase antibody
    See all Triosephosphate isomerase primary antibodies
  • Description

    Goat polyclonal to Triosephosphate isomerase
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-LKPE FVDIINAKQ

    , corresponding to C terminal amino acids 237-249 of Human Triosephosphate isomerase, according to NP_000356.

  • Positive control

    • Human liver lysate, HepG2 whole cell lysate (ab7900).

Properties

Applications

Our Abpromise guarantee covers the use of ab28760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
  • Cellular localization

    Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
  • Database links

  • Alternative names

    • epididymis secretory protein Li 49 antibody
    • HEL-S-49 antibody
    • MGC88108 antibody
    • TIM antibody
    • TPI 1 antibody
    • TPI antibody
    • TPI1 antibody
    • TPID antibody
    • Triose phosphate isomerase 1 antibody
    • Triose phosphate isomerase antibody
    • Triosephosphate isomerase 1 antibody
    • Triosephosphate isomerase antibody
    see all

Images

  • Anti-Triosephosphate isomerase antibody (ab28760) at 0.01 µg/ml + human liver lysate 30µg total protein per lane

    Predicted band size: 26 kDa
    Observed band size: 26 kDa




    Primary incubated for 1 hour.
    Detected by chemiluminescence.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining Triosephosphate isomerase with ab28760 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a Tris/EDTA buffer (pH 9).

References

This product has been referenced in:

  • Zhang CS  et al. Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Nature 548:112-116 (2017). Read more (PubMed: 28723898) »
  • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 01 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
10 µg
Specification
HCT116
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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