Product nameAnti-Triosephosphate isomerase antibody [EPR12149(B)]
See all Triosephosphate isomerase primary antibodies
DescriptionRabbit monoclonal [EPR12149(B)] to Triosephosphate isomerase
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Triosephosphate isomerase aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P60174
- Fetal liver, HeLa and 293T lysates; 293T cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 31 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceTriosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
Cellular localizationCytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
- epididymis secretory protein Li 49 antibody
- HEL-S-49 antibody
- MGC88108 antibody
Flow cytometric analysis of permeabilized 293T cells labeling Triosephosphate isomerase with ab170894 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
All lanes : Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa