Recombinant
RabMAb

Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)

Overview

  • Product name
    Anti-Triosephosphate isomerase antibody [EPR12149(B)]
    See all Triosephosphate isomerase primary antibodies
  • Description
    Rabbit monoclonal [EPR12149(B)] to Triosephosphate isomerase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Triosephosphate isomerase aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P60174

  • Positive control
    • Fetal liver, HeLa and 293T lysates; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Relevance
      Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
    • Cellular localization
      Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
    • Database links
    • Alternative names
      • epididymis secretory protein Li 49 antibody
      • HEL-S-49 antibody
      • MGC88108 antibody
      • TIM antibody
      • TPI 1 antibody
      • TPI antibody
      • TPI1 antibody
      • TPID antibody
      • Triose phosphate isomerase 1 antibody
      • Triose phosphate isomerase antibody
      • Triosephosphate isomerase 1 antibody
      • Triosephosphate isomerase antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized 293T cells labeling Triosephosphate isomerase with ab170894 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    • All lanes : Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    References

    This product has been referenced in:
    • Del Rey MJ  et al. Hif-1a Knockdown Reduces Glycolytic Metabolism and Induces Cell Death of Human Synovial Fibroblasts Under Normoxic Conditions. Sci Rep 7:3644 (2017). WB ; Human . Read more (PubMed: 28623342) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170894.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up