Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Triosephosphate isomerase antibody [EPR12149(B)]
See all Triosephosphate isomerase primary antibodies -
DescriptionRabbit monoclonal [EPR12149(B)] to Triosephosphate isomerase
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Triosephosphate isomerase aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P60174 -
Positive control
- Fetal liver, HeLa and 293T lysates; 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12149(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | 1/1000 - 1/5000. Predicted molecular weight: 31 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceTriosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
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Cellular localizationCytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.
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Database links
- Entrez Gene: 7167 Human
- Entrez Gene: 21991 Mouse
- Entrez Gene: 24849 Rat
- Omim: 190450 Human
- SwissProt: P60174 Human
- SwissProt: P17751 Mouse
- SwissProt: P48500 Rat
- Unigene: 524219 Human
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Alternative names
- epididymis secretory protein Li 49 antibody
- HEL-S-49 antibody
- MGC88108 antibody
see all
Images
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![Flow Cytometry - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)](https://www.abcam.com/ps/products/170/ab170894/Images/ab170894-170774-1708942.JPG)
Flow Cytometry - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)Flow cytometric analysis of permeabilized 293T cells labeling Triosephosphate isomerase with ab170894 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
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![Western blot - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)](https://www.abcam.com/ps/products/170/ab170894/Images/ab170894-170773-170894.JPG)
Western blot - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)All lanes : Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa
Datasheets and documents
References
This product has been referenced in:
- Del Rey MJ et al. Hif-1a Knockdown Reduces Glycolytic Metabolism and Induces Cell Death of Human Synovial Fibroblasts Under Normoxic Conditions. Sci Rep 7:3644 (2017). WB ; Human . Read more (PubMed: 28623342) »
