RecombinantRabMAb

Recombinant Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)

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Flow Cytometry - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
  • Western blot - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
  • Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12149(B)] to Triosephosphate isomerase
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Triosephosphate isomerase antibody [EPR12149(B)]
    See all Triosephosphate isomerase primary antibodies

  • Description

    Rabbit monoclonal [EPR12149(B)] to Triosephosphate isomerase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Triosephosphate isomerase aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P60174

  • Positive control

    • Fetal liver, HeLa and 293T lysates; 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab170894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Relevance

      Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

    • Cellular localization

      Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space.

    • Database links

      see all

    • Alternative names

      • epididymis secretory protein Li 49 antibody
      • HEL-S-49 antibody
      • MGC88108 antibody
      • TIM antibody
      • TPI 1 antibody
      • TPI antibody
      • TPI1 antibody
      • TPID antibody
      • Triose phosphate isomerase 1 antibody
      • Triose phosphate isomerase antibody
      • Triosephosphate isomerase 1 antibody
      • Triosephosphate isomerase antibody
      see all

    Images

    • Flow Cytometry - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
      Flow Cytometry - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)

      Flow cytometric analysis of permeabilized 293T cells labeling Triosephosphate isomerase with ab170894 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    • Western blot - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
      Western blot - Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
      All lanes : Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    • Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)
      Anti-Triosephosphate isomerase antibody [EPR12149(B)] (ab170894)

    References (1)

    Publishing research using ab170894? Please let us know so that we can cite the reference in this datasheet.

    ab170894 has been referenced in 1 publication.

    • Del Rey MJ  et al. Hif-1a Knockdown Reduces Glycolytic Metabolism and Induces Cell Death of Human Synovial Fibroblasts Under Normoxic Conditions. Sci Rep 7:3644 (2017). WB ; Human . PubMed: 28623342

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