Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 4 - 6 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 53 kDa. additonal band of unkown identity was also concistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide.|
FunctionInhibits activation of NF-kappa-B mediated by TNF.
Sequence similaritiesContains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region.
- Information by UniProt
- RING finger protein 206 antibody
- RNF206 antibody
- TRAF Interacting Protein antibody
Anti-TRIP antibody (ab4533) at 2 µg/ml + Human Testis lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
In paraffin embedded Human Spleen shows perinucxlear and textured cystoplasmic staining in selective areas.ab4533 (4µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
ab4533 has not yet been referenced specifically in any publications.