Anti-TRIP12/ULF antibody (ab86220)
Key features and details
- Rabbit polyclonal to TRIP12/ULF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIP12/ULF antibody
See all TRIP12/ULF primary antibodies -
Description
Rabbit polyclonal to TRIP12/ULF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant -
Immunogen
Synthetic peptide corresponding to Human TRIP12/ULF.
Database link: NP_004229.1 -
Positive control
- HeLa whole cell lysate (ab150035)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 220 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 220 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1. -
Database links
- Entrez Gene: 9320 Human
- Omim: 604506 Human
- SwissProt: Q14669 Human
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Alternative names
- KIAA0045 antibody
- MGC138849 antibody
- MGC138850 antibody
see all
Images
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All lanes : Anti-TRIP12/ULF antibody (ab86220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 1.5 µg
Lane 3 : HeLa whole cell lysate at 0.5 µg
Developed using the ECL technique.
Predicted band size: 220 kDa
Exposure time: 3 minutes -
Detection of human TRIP12/ULF in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab86220 has been referenced in 5 publications.
- Molkentine DP et al. p16 Represses DNA Damage Repair via a Novel Ubiquitin-Dependent Signaling Cascade. Cancer Res 82:916-928 (2022). PubMed: 34965932
- Harris LD et al. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. J Biol Chem 296:100246 (2021). PubMed: 33853758
- Cipolla L et al. UBR5 interacts with the replication fork and protects DNA replication from DNA polymerase ? toxicity. Nucleic Acids Res 47:11268-11283 (2019). PubMed: 31586398
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Mounir Z et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . PubMed: 27183006