Key features and details
- Rabbit polyclonal to TRIP12/ULF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIP12/ULF antibody
See all TRIP12/ULF primary antibodies
DescriptionRabbit polyclonal to TRIP12/ULF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
Synthetic peptide corresponding to Human TRIP12/ULF.
Database link: NP_004229.1
- HeLa whole cell lysate (ab150035)
This product was previously labelled as TRIP12
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 220 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceTRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
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All lanes : Anti-TRIP12/ULF antibody (ab86220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 1.5 µg
Lane 3 : HeLa whole cell lysate at 0.5 µg
Developed using the ECL technique.
Predicted band size: 220 kDa
Exposure time: 3 minutes
Detection of human TRIP12/ULF in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab86220 has been referenced in 3 publications.
- Cipolla L et al. UBR5 interacts with the replication fork and protects DNA replication from DNA polymerase ? toxicity. Nucleic Acids Res 47:11268-11283 (2019). PubMed: 31586398
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Mounir Z et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . PubMed: 27183006