Product nameAnti-TRIP12/ULF antibody
See all TRIP12/ULF primary antibodies
DescriptionRabbit polyclonal to TRIP12/ULF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
Synthetic peptide corresponding to Human TRIP12/ULF.
Database link: NP_004229.1
- HeLa whole cell lysate (ab150035)
This product was previously labelled as TRIP12
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 220 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceTRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
- KIAA0045 antibody
- MGC138849 antibody
- MGC138850 antibody
All lanes : Anti-TRIP12/ULF antibody (ab86220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 1.5 µg
Lane 3 : HeLa whole cell lysate at 0.5 µg
Developed using the ECL technique.
Predicted band size: 220 kDa
Exposure time: 3 minutes
Detection of human TRIP12/ULF in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). Read more (PubMed: 28689657) »
- Mounir Z et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . Read more (PubMed: 27183006) »