Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIP12/ULF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human TRIP12/ULF.
    Database link: NP_004229.1

  • Positive control

  • General notes

     This product was previously labelled as TRIP12

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 220 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance

    TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
  • Database links

  • Alternative names

    • KIAA0045 antibody
    • MGC138849 antibody
    • MGC138850 antibody
    • Thyroid hormone receptor interactor 12 antibody
    • Thyroid receptor interacting protein 12 antibody
    • TRIP 12 antibody
    see all

Images

  • All lanes : Anti-TRIP12/ULF antibody (ab86220) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 5 µg
    Lane 2 : HeLa whole cell lysate at 1.5 µg
    Lane 3 : HeLa whole cell lysate at 0.5 µg

    Developed using the ECL technique.

    Predicted band size: 220 kDa


    Exposure time: 3 minutes
  • Detection of human TRIP12/ULF in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

  • Flack JE  et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). Read more (PubMed: 28689657) »
  • Mounir Z  et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. Elife 5:N/A (2016). WB . Read more (PubMed: 27183006) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

We do not have data for mouse reactivity for this TRIP12 antibody, ab86220. The amino acid sequence of the immunogen that generated the antibody is well-conserved in the mouse protein so cross-reaction is possible, though not experimentally confirmed.

Read More

Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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