Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIP13/PCH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Synthetic peptide corresponding to Human TRIP13/PCH2 aa 1-50 (N terminal).
    Database link: NP_004228.1

  • Positive control

    • HeLa, Jurkat and 293T whole cell lysates.
  • General notes

     This product was previously labelled as TRIP13

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab128153 was affinity purified using an epitope specific to TRIP13/PCH2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 16E1-BP antibody
    • 16E1BP antibody
    • Homo sapiens HPV16 E1 protein binding protein mRNA complete cds antibody
    • HPV16 E1 protein binding protein antibody
    • HPV16 E1 protein-binding protein antibody
    • Human papillomavirus type 16 E1 protein binding protein antibody
    • Human papillomavirus type 16 E1 protein-binding protein antibody
    • Pachytene checkpoint protein 2 homolog antibody
    • PCH2 antibody
    • Thyroid hormone receptor interactor 13 antibody
    • Thyroid receptor interacting protein 13 antibody
    • Thyroid receptor-interacting protein 13 antibody
    • TR-interacting protein 13 antibody
    • TRIP-13 antibody
    • Trip13 antibody
    • TRP13_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRIP13/PCH2 antibody (ab128153) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 30 seconds
  • Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
    ab128153 at 1µg/ml staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128153 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab128153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
45 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 09 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up