Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIP13/PCH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Pig, Chimpanzee, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human TRIP13/PCH2 aa 50-100.
    Database link: NP_004228.1

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899)
  • General notes

     This product was previously labelled as TRIP13

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 16E1-BP antibody
    • 16E1BP antibody
    • Homo sapiens HPV16 E1 protein binding protein mRNA complete cds antibody
    • HPV16 E1 protein binding protein antibody
    • HPV16 E1 protein-binding protein antibody
    • Human papillomavirus type 16 E1 protein binding protein antibody
    • Human papillomavirus type 16 E1 protein-binding protein antibody
    • Pachytene checkpoint protein 2 homolog antibody
    • PCH2 antibody
    • Thyroid hormone receptor interactor 13 antibody
    • Thyroid receptor interacting protein 13 antibody
    • Thyroid receptor-interacting protein 13 antibody
    • TR-interacting protein 13 antibody
    • TRIP-13 antibody
    • Trip13 antibody
    • TRP13_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRIP13/PCH2 antibody (ab128171) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 49 kDa


    Exposure time: 3 minutes
  • Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
    a128171, at 1 µg/ml, staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128171 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG.
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:

  • Yost S  et al. Biallelic TRIP13 mutations predispose to Wilms tumor and chromosome missegregation. Nat Genet 49:1148-1151 (2017). WB ; Human . Read more (PubMed: 28553959) »
See 1 Publication for this product

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