Key features and details
- Rabbit polyclonal to TRIP13/PCH2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIP13/PCH2 antibody
See all TRIP13/PCH2 primary antibodies
DescriptionRabbit polyclonal to TRIP13/PCH2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human TRIP13/PCH2 aa 382-432.
Database link: NP_004228.1
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
This product was previously labelled as TRIP13
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the AAA ATPase family.
- Information by UniProt
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All lanes : Anti-TRIP13/PCH2 antibody (ab128187) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 3 minutes
Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
Lane 1: ab128187 at 1µg/ml staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128187 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
ab128187 has not yet been referenced specifically in any publications.