Product nameAnti-TRIP13/PCH2 antibody
See all TRIP13/PCH2 primary antibodies
DescriptionRabbit polyclonal to TRIP13/PCH2
Detects endogenous levels of total TRIP13/PCH2 protein.
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human TRIP13/PCH2 (C terminal).
- Extracts from LOVO cells and NIH 3T3 cells IHC-P: Human lunf FFPE tissue sections.
This product was previously labelled as TRIP13
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
Sequence similaritiesBelongs to the AAA ATPase family.
- Information by UniProt
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IHC image of TRIP13/PCH2 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-TRIP13/PCH2 antibody (ab64964) at 1/500 dilution
Lane 1 : extracts from LOVO cells without immunizing peptide
Lane 2 : extract from NIH 3T3 cells, without immunizing peptide
Lane 3 : extract from NIH 3T3 cells with immunizing peptide
Predicted band size: 49 kDa
Observed band size: 49 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.