• Product name
  • Description
    Rabbit polyclonal to TRIP15
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to TRIP15. The epitope recognized by ab10426 maps to a region between residues 1 and 50 of human TRIP15.
    Database link: P61201

  • Positive control
    • Purchase matching WB positive control:Recombinant TRIP15 protein
    • Whole cell lysate from mock transfected or CSN2 transfected HEK293T cells. IHC human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer IHC mouse controls: Squamous Cell Carcinoma
  • General notes

    CSN2 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.



Our Abpromise guarantee covers the use of ab10426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
  • Sequence similarities
    Belongs to the CSN2 family.
    Contains 1 PCI domain.
  • Post-translational
    Phosphorylated by CK2 and PKD kinases.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ALIEN antibody
    • Alien Drosophila himolog of antibody
    • Alien homolog antibody
    • COP 9 antibody
    • COP9 antibody
    • COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) antibody
    • COP9 constitutive photomorphogenic homolog subunit 2 antibody
    • COP9 signalosome complex subunit 2 antibody
    • COPS 2 antibody
    • Cops2 antibody
    • CSN2 antibody
    • CSN2_HUMAN antibody
    • JAB1 containing signalosome subunit 2 antibody
    • JAB1-containing signalosome subunit 2 antibody
    • SGN 2 antibody
    • SGN2 antibody
    • Signalosome subunit 2 antibody
    • Thyroid Hormone Receptor Interactor15 antibody
    • Thyroid Hormone Receptor Interactor 15 antibody
    • Thyroid receptor-interacting protein 15 antibody
    • TR-interacting protein 15 antibody
    • TRIP 15 antibody
    • TRIP-15 antibody
    • TRIP15 antibody
    see all


  • Whole cell lysate (50 μg - E; 10 μg - T) from mock transfected (E) or TRIP15 transfected (T) HEK293T cells. Antibody used at the indicated concentration.


  • IHC-P section of human prostate carcinoma (left) and mouse squamous cell carcinoma (right).

    Antibody used at a dilution of 1:5,000 (0.2μg/ml) and 1:1,000 (1μg/ml).

    Detection: DAB


This product has been referenced in:
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your enquiry. May I also thank you for explaining the problem so well. The images that you e-mailed are beautiful and clearly highlight the problems that you have been having. We often observe LOT variation in our antibodies. This can ...

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