Anti-TRIP15 antibody (ab155774)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-TRIP15 antibody
See all TRIP15 primary antibodies -
DescriptionRabbit polyclonal to TRIP15
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 381-443 of Human TRIP15 (P61201).
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Positive control
- Purchase matching WB positive control:Recombinant TRIP15 protein
- A431, H1299 and HeLa whole cell lysate; HepG2 cell line; HeLa cells; Human Mahlavu xenograft tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 52 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionEssential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.
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Sequence similaritiesBelongs to the CSN2 family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated by CK2 and PKD kinases. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 430917 Chicken
- Entrez Gene: 9318 Human
- Entrez Gene: 12848 Mouse
- Entrez Gene: 100125956 Pig
- Entrez Gene: 261736 Rat
- Entrez Gene: 548537 Xenopus tropicalis
- Omim: 604508 Human
- SwissProt: P61201 Human
see all -
Alternative names
- ALIEN antibody
- Alien Drosophila himolog of antibody
- Alien homolog antibody
see all
Images
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Western blot - Anti-TRIP15 antibody (ab155774)All lanes : Anti-TRIP15 antibody (ab155774) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TRIP15 antibody (ab155774)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling TRIP15 with ab155774 at 1/500 dilution. Blue: Hoechst 33342 staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP15 antibody (ab155774)Immunohistochemical analysis of paraffin-embedded Human Mahlavu xenograft tissue, labeling TRIP15 with ab155774 at 1/100 dilution.
Protocols
References
This product has been referenced in:
- Liu J et al. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity. Leukemia N/A:N/A (2018). Read more (PubMed: 30026574) »
