Key features and details
- Rabbit polyclonal to TRIP6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIP6 antibody
See all TRIP6 primary antibodies
DescriptionRabbit polyclonal to TRIP6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment, corresponding to a region within amino acids 162-454 of Human TRIP6 (Uniprot: Q15654).
- Purchase matching WB positive control:Recombinant Human TRIP6 protein
- 293T, A431, H1299, HeLa and HepG2 whole cell lysates; HeLa cells; Human TOV21G xenograft tissue.
General notesKeep as concentrated solution.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionRelays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
Tissue specificityAbundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors.
Sequence similaritiesBelongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains.
DomainThe LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI.
modificationsPhosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion. Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- MGC10556 antibody
- MGC10558 antibody
- MGC29959 antibody
Anti-TRIP6 antibody (ab137478) at 1/500 dilution + A. Hela whole cell lysate/extract at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed (4%, 15mins) HeLa cells labelling TRIP6 with ab137478 at 1/500 dilution. The image on the right is co-stained with Hoechst 33343.
Immunohistochemical analysis of paraffin embedded Human TOV21G xenograft tissue labelling TRIP6 with ab137478 at 1/500 dilution.
ab137478 has been referenced in 2 publications.
- Zhao X et al. TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/ß-catenin. Cancer Cell Int 20:51 (2020). PubMed: 32082081
- Zhu L et al. TRIP6 functions as a potential oncogene and facilitated proliferation and metastasis of gastric cancer. Biologics 13:101-110 (2019). PubMed: 31354238