Key features and details
- Rabbit polyclonal to TRIP6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIP6 antibody
See all TRIP6 primary antibodies
DescriptionRabbit polyclonal to TRIP6
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1 and 50 of human TRIP6 (NP_003293.1)
- Purchase matching WB positive control:Recombinant Human TRIP6 protein
- Whole cell lysate from HeLa or 293T cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRelays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
Tissue specificityAbundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors.
Sequence similaritiesBelongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains.
DomainThe LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI.
modificationsPhosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion. Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- MGC10556 antibody
- MGC10558 antibody
- MGC29959 antibody
All lanes : Anti-TRIP6 antibody (ab70747) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
1mg whole cell lysate from HeLa cells was immunprecipitated using ab70747 at 3µg/mg of lysate. For the subsequent western blot, 20% of the immunoprecipitate was probed with ab70747 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
ab70747 has been referenced in 1 publication.
- Gou H et al. TTPAL Promotes Colorectal Tumorigenesis by Stabilizing TRIP6 to Activate Wnt/ß-Catenin Signaling. Cancer Res 79:3332-3346 (2019). PubMed: 31018940