Product nameAnti-Tripeptidyl peptidase II/TPPII antibody
See all Tripeptidyl peptidase II/TPPII primary antibodies
DescriptionRabbit polyclonal to Tripeptidyl peptidase II/TPPII
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human Tripeptidyl peptidase II/TPPII aa 1-300.
MATAATEEPFPFHGLLPKKETGAASFLCRYPEYDGRGVLIAVLDTGVDPG APGMQVTTDGKPKIVDIIDTTGSGDVNTATEVEPKDGEIVGLSGRVLKIP ASWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPVHRVALAEAC RKQEEFDVANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVW HDGEVWRACIDSNEDGDLSKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYD DGNLLSIVTSGGAHGTHVASIAAGHFPEEPERNGVAPGAQILSIKIGDTR
Database link: P29144
- WB: HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human testis tissue.
This product was previously labelled as Tripeptidyl peptidase II
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).|
|IHC-P||1/20 - 1/200.|
RelevanceTripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
- TPP II antibody
- TPP2 antibody
- Tripeptidyl aminopeptidase antibody
Paraffin-embedded human testis tissue stained for Tripeptidyl peptidase II/TPPII with ab224609 (1/100 dilution) in immunohistochemical analysis.
All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody (ab224609) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 138 kDa
Observed band size: 138 kDa
ab224609 has not yet been referenced specifically in any publications.