Recombinant Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
![Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)](https://www.abcam.com/ps/products/180/ab180177/Images/ab180177-208678-anti-tripeptidyl-peptidase-ii-antibody-epr13202-western-blot.jpg "Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
See all Tripeptidyl peptidase II/TPPII primary antibodies -
Description
Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
Database link: P29144 -
Positive control
- Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13202 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
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| ICC/IF |
1/100 - 1/250.
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa). |
|
ICC/IF
1/100 - 1/250. |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Tripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 7174 Human
- Omim: 190470 Human
- SwissProt: P29144 Human
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Alternative names
- TPP II antibody
- TPP2 antibody
- Tripeptidyl aminopeptidase antibody
see all
Images
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Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 138 kDa
Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands. -
![Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)](https://www.abcam.com/ps/products/180/ab180177/Images/ab180177-208682-anti-tripeptidyl-peptidase-ii-antibody-epr13202-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).
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![Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)](https://www.abcam.com/ps/products/180/ab180177/Images/ab180177-208680-anti-tripeptidyl-peptidase-ii-antibody-epr13202-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)](https://www.abcam.com/ps/products/180/ab180177/Images/ab180177-208679-anti-tripeptidyl-peptidase-ii-antibody-epr13202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)](https://www.abcam.com/ps/products/180/ab180177/Images/ab180177-5-benefits-of-recombinant-antibodies.png)
Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab180177 has been referenced in 1 publication.
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233
