Product nameAnti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
See all Tripeptidyl peptidase II/TPPII primary antibodies
DescriptionRabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
Database link: P29144
- Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceTripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
- TPP II antibody
- TPP2 antibody
- Tripeptidyl aminopeptidase antibody
All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 138 kDa
Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab180177 has not yet been referenced specifically in any publications.