RecombinantRabMAb

Recombinant Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
    See all Tripeptidyl peptidase II/TPPII primary antibodies

  • Description

    Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
    Database link: P29144

  • Positive control

    • Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.

  • General notes

     This product was previously labelled as Tripeptidyl peptidase II

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab180177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      Tripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • TPP II antibody
        • TPP2 antibody
        • Tripeptidyl aminopeptidase antibody
        • Tripeptidyl peptidase 2 antibody
        • Tripeptidyl peptidase II antibody
        see all

      Images

      • Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
        Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
        All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution

        Lane 1 : Jurkat lysate
        Lane 2 : HeLa lysate
        Lane 3 : HepG2 lysate
        Lane 4 : fetal liver lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 138 kDa
        Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.

      • Flow Cytometry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
        Flow Cytometry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

        Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).

      • Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
        Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

        Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

        Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
        Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

      References (1)

      Publishing research using ab180177? Please let us know so that we can cite the reference in this datasheet.

      ab180177 has been referenced in 1 publication.

      • Tang M  et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233

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