Recombinant
RabMAb

Recombinant Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

Overview

  • Product name

    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
    See all Tripeptidyl peptidase II/TPPII primary antibodies
  • Description

    Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
    Database link: P29144

  • Positive control

    • Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
  • General notes

     This product was previously labelled as Tripeptidyl peptidase II

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Tripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • TPP II antibody
      • TPP2 antibody
      • Tripeptidyl aminopeptidase antibody
      • Tripeptidyl peptidase 2 antibody
      • Tripeptidyl peptidase II antibody
      see all

    Images

    • All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : HeLa lysate
      Lane 3 : HepG2 lysate
      Lane 4 : fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 138 kDa
      Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).

    • Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab180177 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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