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  1. Link

    tripeptidyl-peptidase-iitppii-antibody-epr13202-ab180177.pdf

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Immunology Adaptive Immunity MHC Class I
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RecombinantRabMAb

Recombinant Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

  • Datasheet
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Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
  • Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
  • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
    See all Tripeptidyl peptidase II/TPPII primary antibodies
  • Description

    Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
    Database link: P29144

  • Positive control

    • Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13202
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Associated products

  • Alternative Versions

    • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for IP.

Target

  • Relevance

    Tripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 7174 Human
    • Omim: 190470 Human
    • SwissProt: P29144 Human
    • Alternative names

      • TPP II antibody
      • TPP2 antibody
      • Tripeptidyl aminopeptidase antibody
      • Tripeptidyl peptidase 2 antibody
      • Tripeptidyl peptidase II antibody
      see all

    Images

    • Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
      Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
      All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : HeLa lysate
      Lane 3 : HepG2 lysate
      Lane 4 : fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 138 kDa
      Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
      Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

      Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
      Immunocytochemistry/ Immunofluorescence - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

      Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

      Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
      Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab180177? Please let us know so that we can cite the reference in this datasheet.

    ab180177 has been referenced in 1 publication.

    • Tang M  et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233

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