Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
See all Tripeptidyl peptidase II/TPPII primary antibodies
DescriptionRabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
Database link: P29144
- Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
This product was previously labelled as Tripeptidyl peptidase II
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceTripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
- TPP II antibody
- TPP2 antibody
- Tripeptidyl aminopeptidase antibody
All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 138 kDa
Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab180177 has been referenced in 1 publication.
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233