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RecombinantRabMAb

Recombinant Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)

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Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
  • Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
  • Immunocytochemistry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
  • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free
    See all Tripeptidyl peptidase II/TPPII primary antibodies

  • Description

    Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)more details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
    Database link: P29144

  • General notes

    ab250179 is the carrier-free version of ab180177.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP.

Target

  • Relevance

    Tripeptidyl peptidase II is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It may be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Tripeptidyl peptidase II is a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • TPP II antibody
      • TPP2 antibody
      • Tripeptidyl aminopeptidase antibody
      • Tripeptidyl peptidase 2 antibody
      • Tripeptidyl peptidase II antibody
      see all

    Images

    • Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Fetal liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 138 kDa
      Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.



      This data was developed using ab180177, the same antibody clone in a different buffer formulation.

    • Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      Flow Cytometry (Intracellular) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)

      This data was developed using ab180177, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green). 

       

       

    • Immunocytochemistry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      Immunocytochemistry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      This data was developed using ab180177, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      This data was developed using ab180177, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
      Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250179? Please let us know so that we can cite the reference in this datasheet.

    ab250179 has not yet been referenced specifically in any publications.

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