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Abcam's TripleStain IHC kit (ab183286) is designed to use with user supplied two mouse primary antibodies and one rabbit primary antibody to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue only; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. This kit supplies polymer enzyme conjugates: Rabbit HRP Polymer, Mouse HRP Polymer and Mouse AP Polymer with three chromogens, DAB Chromogen (brown); Permanent Red Chromogen (red); and Emerald Chromogen (green). Abcam's TripleStain IHC kit (ab183286) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.
|Components||Identifier||24 ml||72 ml||240 ml||—|
|Antibody Blocker (40x)||2 x 15ml||1 x 50ml||1 x 100ml|
|Blocker A||1 x 12ml||2 x 18ml||1 x 120ml|
|Blocker B||1 x 12ml||2 x 18ml||1 x 120ml|
|DAB Chromogen (20x)||1 x 1.5ml||1 x 2ml||1 x 6ml|
|DAB Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Emerald Chromogen||1 x 15ml||2 x 18ml||1 x 120ml|
|Mouse AP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Mouse HRP Polymer||1 x 12ml||2 x 18ml||1 x 120ml|
|Non-aqueous Mounting Medium||1 x 12ml||2 x 18ml|
|Permanent Red Activator (5x)||1 x 3ml||1 x 7.2ml||2 x 12ml|
|Permanent Red Chromogen (100x)||1 x 150µl||1 x 360µl||1 x 1.2ml|
|Permanent Red Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Rabbit HRP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
Our Abpromise guarantee covers the use of ab183286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab183286 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"