TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green) (ab183286)
Overview
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Product name
TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green) -
Assay type
Polymer Multiplex -
Product overview
Abcam's TripleStain IHC kit (ab183286) is designed to use with user supplied two mouse primary antibodies and one rabbit primary antibody to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue only; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. This kit supplies polymer enzyme conjugates: Rabbit HRP Polymer, Mouse HRP Polymer and Mouse AP Polymer with three chromogens, DAB Chromogen (brown); Permanent Red Chromogen (red); and Emerald Chromogen (green). Abcam's TripleStain IHC kit (ab183286) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip. -
Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 72 ml 60 ml 6 ml 24 ml Antibody Blocker (40x) 1 x 100ml 1 x 100ml 1 x 30ml 1 x 30ml Blocker A 2 x 18ml 1 x 120ml 1 x 6ml 1 x 12ml Blocker B 2 x 18ml 1 x 120ml 1 x 6ml 1 x 12ml DAB Chromogen (20x) 1 x 2ml 1 x 6ml 1 x 1.5ml 1 x 1.5ml DAB Substrate 2 x 18ml 1 x 120ml 1 x 15ml 1 x 15ml Emerald Chromogen 2 x 18ml 1 x 120ml 1 x 6ml 1 x 15ml Mouse AP Polymer 1 x 18ml 1 x 60ml 1 x 6ml 1 x 6ml Mouse HRP Polymer 2 x 18ml 1 x 60ml 1 x 6ml 1 x 12ml Non-aqueous Mounting Medium 1 x 9ml 0 x 0ml 1 x 6ml 1 x 3ml Permanent Red Activator (5x) 1 x 7.2ml 1 x 24ml 1 x 3ml 1 x 3ml Permanent Red Chromogen (100x) 1 x 360µl 1 x 1.2ml 1 x 150µl 1 x 150µl Permanent Red Substrate 2 x 18ml 1 x 120ml 1 x 15ml 1 x 15ml Rabbit HRP Polymer 1 x 18ml 1 x 60ml 1 x 6ml 1 x 6ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab183286 has been referenced in 5 publications.
- Reagan-Steiner S et al. Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta. Emerg Infect Dis 28:510-517 (2022). PubMed: 35138244
- Mathiasen SL et al. Bacterial genotoxins induce T cell senescence. Cell Rep 35:109220 (2021). PubMed: 34107253
- Zadka Ł et al. Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Select Colon Disorders. Am J Pathol 191:2147-2171 (2021). PubMed: 34428422
- Papalampros A et al. Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPa Expression. Cancers (Basel) 12:N/A (2020). PubMed: 32645996
- Cui G et al. Up-regulation of FGF15/19 signaling promotes hepatocellular carcinoma in the background of fatty liver. J Exp Clin Cancer Res 37:136 (2018). PubMed: 29973237