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The TripleStain IHC kit (ab183292) is designed to use with two mouse antibodies and one rabbit antibody (user supplied) to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested in paraffin-embedded formalin-fixed tissues. However, it can be used on frozen specimens and freshly prepared monolayer cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-mouse IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). DAB (Brown) and Permanent Red (red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. A Primer step is used to increase specificity of antibody staining. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system.
|Components||Identifier||24 ml||72 ml||240 ml||—|
|Antibody Blocker (40x)||2 x 15ml||1 x 50ml||1 x 100ml|
|Blocker A||1 x 12ml||2 x 18ml||1 x 120ml|
|Blocker B||1 x 12ml||2 x 18ml||1 x 120ml|
|DAB Chromogen (20x)||1 x 1.5ml||1 x 2ml||1 x 6ml|
|DAB Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Emerald Chromogen||1 x 15ml||2 x 18ml||1 x 120ml|
|Mouse AP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Mouse HRP Polymer||1 x 12ml||2 x 18ml||1 x 120ml|
|Mouse Primer||1 x 12ml||2 x 18ml||1 x 120ml|
|Non-aqueous Mounting Medium||1 x 12ml||2 x 18ml|
|Permanent Red Activator (5x)||1 x 3ml||1 x 7.2ml||2 x 12ml|
|Permanent Red Chromogen (100x)||1 x 150µl||1 x 360µl||1 x 1.2ml|
|Permanent Red Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Rabbit HRP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
Our Abpromise guarantee covers the use of ab183292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab183292 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"