Overview

  • Product name

    TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni)
  • Assay type

    Polymer Multiplex
  • Product overview

    Abcam's TripleStain IHC Kit (ab183291) is designed to be used with user-supplied goat, mouse and rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue; however it may also be used on frozen or freshly prepared monolayer cell smears.

    Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183291) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and DAB-Ni (black). Abcam's TripleStain IHC Kit (ab183291) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the users to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 24 ml 72 ml 240 ml
    DAB Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 6ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    DAB-Ni Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Goat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml
    Nickel Solution (7x) 1 x 3ml 1 x 6ml 1 x 18ml
    Non-aqueous Mounting Medium 1 x 15ml 2 x 18ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Triple Stain Blocker 1 x 12ml 2 x 18ml 1 x 120ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Protocols

References

ab183291 has not yet been referenced specifically in any publications.

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