Product nameTripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni)
Assay typePolymer Multiplex
Abcam's TripleStain IHC Kit (ab183291) is designed to be used with user-supplied goat, mouse and rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183291) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and DAB-Ni (black). Abcam's TripleStain IHC Kit (ab183291) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the users to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 24 ml 72 ml 240 ml — DAB Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 6ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml DAB-Ni Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml Goat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml Nickel Solution (7x) 1 x 3ml 1 x 6ml 1 x 18ml Non-aqueous Mounting Medium 1 x 15ml 2 x 18ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Triple Stain Blocker 1 x 12ml 2 x 18ml 1 x 120ml
Our Abpromise guarantee covers the use of ab183291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183291 has not yet been referenced specifically in any publications.