Product nameTripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green)
Assay typePolymer Multiplex
Abcam's TripleStain IHC Kit (ab183290) is designed to be used with user-supplied one goat, one mouse and one rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissur; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183290) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183290) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the user to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 24 ml 72 ml 240 ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml Goat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml Non-aqueous Mounting Medium 1 x 12ml 2 x 18ml 2 x 30ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Plastic pipette 1 unit 1 unit 1 unit Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Triple Stain Blocker 1 x 12ml 2 x 18ml 1 x 120ml
Our Abpromise guarantee covers the use of ab183290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183290 has not yet been referenced specifically in any publications.