TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red) (ab183297)

Overview

  • Product name

    TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)
  • Assay type

    Polymer Multiplex
  • Product overview

    The TripleStain IHC kit (ab183297) is designed to be used with user supplied mouse, rat and rabbit primary antibodies to detect three distinct antigens on mouse tissue and cell samples or human tissue. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen tissue or cell smears.


    Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-rat IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the mouse and rat host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). This protocol also includes a method to dehydrate, clear and permanently mount slides with coverslip.

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 24 ml 72 ml 240 ml
    Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml
    Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml
    Non-aqueous Mounting Medium 1 x 12ml 2 x 18ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Plastic pipette 1 unit 1 unit 1 unit
    Rabbit HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rat Primer 1 x 12ml 2 x 18ml 1 x 120ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183297 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

TripleStain IHC Kit: M&R&Rt

Excellent Excellent 5/5 (Ease of Use)
Abreviews
A nice kit. It is easy to use and the instructions are easy to handle and to follow.
These are beautiful clear color results that you can also optically separate from each other. We used the kit on paraffin samples and performed a hematoxylin counterstain.

Abcam user community

Verified customer

Submitted Aug 29 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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