Product nameTripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)
Assay typePolymer Multiplex
The TripleStain IHC kit (ab183297) is designed to be used with user supplied mouse, rat and rabbit primary antibodies to detect three distinct antigens on mouse tissue and cell samples or human tissue. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen tissue or cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-rat IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the mouse and rat host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). This protocol also includes a method to dehydrate, clear and permanently mount slides with coverslip.
Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 24 ml 72 ml 240 ml — Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml Mouse HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml Non-aqueous Mounting Medium 1 x 12ml 2 x 18ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Plastic pipette 1 unit 1 unit 1 unit Rabbit HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rat Primer 1 x 12ml 2 x 18ml 1 x 120ml
Our Abpromise guarantee covers the use of ab183297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab183297 has not yet been referenced specifically in any publications.