TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red) (ab183297)
Overview
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Product name
TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red) -
Assay type
Polymer Multiplex -
Product overview
The TripleStain IHC kit (ab183297) is designed to be used with user supplied mouse, rat and rabbit primary antibodies to detect three distinct antigens on mouse tissue and cell samples or human tissue. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen tissue or cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-rat IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the mouse and rat host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). This protocol also includes a method to dehydrate, clear and permanently mount slides with coverslip.
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Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 60 ml 6 ml 18 ml Antibody Blocker (40x) 1 x 125ml 1 x 50ml 1 x 30ml DAB Chromogen (20x) 1 x 6ml 1 x 1.5ml 1 x 2ml DAB Substrate 1 x 120ml 1 x 12ml 1 x 36ml Emerald Chromogen 1 x 60ml 1 x 6ml 1 x 18ml Mouse HRP Polymer 1 x 60ml 1 x 6ml 1 x 18ml Non-aqueous Mounting Medium 0 x 0ml 1 x 6ml 1 x 18ml Permanent Red Activator (5x) 1 x 24ml 1 x 3ml 1 x 7.2ml Permanent Red Chromogen (100x) 1 x 1.2ml 1 x 150µl 1 x 360µl Permanent Red Substrate 1 x 120ml 1 x 15ml 1 x 36ml Rabbit HRP Polymer 1 x 60ml 1 x 6ml 1 x 18ml Rat AP Polymer 1 x 60ml 1 x 6ml 1 x 18ml Rat Primer 1 x 60ml 1 x 6ml 1 x 18ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183297 has not yet been referenced specifically in any publications.