Product nameTripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & DAB/Ni)
Assay typePolymer Multiplex
Abcam's TripleStain IHC Kit (ab183289) is designed to be used with user-supplied one mouse primary antibody and two rabbit primary antibodies to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it also can also be used on frozen tissue or cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183289) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and DAB-Ni (Black). Abcam's TripleStain IHC Kit (ab183289) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow the user to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with coverslip.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 24 ml 72 ml 240 ml Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml Blocker A 1 x 12ml 2 x 18ml 1 x 120ml Blocker B 1 x 12ml 2 x 18ml 1 x 120ml DAB Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 12ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml DAB-Ni Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Nickel Solution (7x) 1 x 3ml 1 x 6ml 1 x 18ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rabbit HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml
Our Abpromise guarantee covers the use of ab183289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183289 has not yet been referenced specifically in any publications.