Overview

  • Product name

    TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & DAB/Ni)
  • Assay type

    Polymer Multiplex
  • Product overview

    Abcam's TripleStain IHC Kit (ab183289) is designed to be used with user-supplied one mouse primary antibody and two rabbit primary antibodies to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it also can also be used on frozen tissue or cell smears.

     

    Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183289) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and DAB-Ni (Black). Abcam's TripleStain IHC Kit (ab183289) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow the user to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with coverslip.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 24 ml 72 ml 240 ml
    Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml
    Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml
    Blocker A 1 x 12ml 2 x 18ml 1 x 120ml
    Blocker B 1 x 12ml 2 x 18ml 1 x 120ml
    DAB Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 12ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    DAB-Ni Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Nickel Solution (7x) 1 x 3ml 1 x 6ml 1 x 18ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rabbit HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Protocols

References

ab183289 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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