TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP) (ab183288)

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Overview

  • Product name

    TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP)

  • Assay type

    Polymer Multiplex

  • Product overview

    Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it can also be used for frozen tissue or cell smears.

     

    Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183288) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183288) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 24 ml 72 ml 240 ml
    —
    Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml
    Blocker A 1 x 12ml 2 x 18ml 1 x 120ml
    Blocker B 1 x 12ml 2 x 18ml 1 x 120ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Non-aqueous Mounting Medium 1 x 12ml 2 x 18ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Plastic pipette 1 unit 1 unit 1 unit
    Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rabbit HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183288 has not yet been referenced specifically in any publications.

Publishing research using ab183288? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question


Inquiry: Can we use ab183288 with autostainers for IHC?

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Answer

Thank you for your enquiry.

We do not have experience on testing ab183288 with autostainers, some of our customers used GBI other polymer detection kits on autostainer, which worked well for them.

One thing to clarify is that ab183288 is a triple staining kit with Emerald chromogen which is water soluble, it requires doing hematoxylin prior to emerald chromogen step, quick wash after stained with emerald chromogen, and air dry before going to dehydration step . Please use fresh ethanol and xylene to dehydrate and clear if staining of either emerald or permanent red fades.

Please let me know if you have any question. Have a great day.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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