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Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it can also be used for frozen tissue or cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183288) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183288) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.
|Components||Identifier||24 ml||72 ml||240 ml||—|
|Antibody Blocker (40x)||2 x 15ml||1 x 50ml||1 x 100ml|
|Blocker A||1 x 12ml||2 x 18ml||1 x 120ml|
|Blocker B||1 x 12ml||2 x 18ml||1 x 120ml|
|DAB Chromogen (20x)||1 x 1.5ml||1 x 2ml||1 x 6ml|
|DAB Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Emerald Chromogen||1 x 15ml||2 x 18ml||1 x 120ml|
|Mouse HRP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Non-aqueous Mounting Medium||1 x 12ml||2 x 18ml|
|Permanent Red Activator (5x)||1 x 3ml||1 x 7.2ml||2 x 12ml|
|Permanent Red Chromogen (100x)||1 x 150µl||1 x 360µl||1 x 1.2ml|
|Permanent Red Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Rabbit AP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Rabbit HRP Polymer||1 x 12ml||2 x 18ml||1 x 120ml|
Our Abpromise guarantee covers the use of ab183288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab183288 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"