Product nameTripleStain IHC Kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green)
Assay typePolymer Multiplex
Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested on tissue specimens that are paraffin embedded; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183295) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit HRP Polymer and Rabbit AP Polymer with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). Abcam's TripleStain IHC Kit (ab183295) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow the user to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with coverslip.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 240 ml 72 ml 24 ml Non-aqueous Mounting Medium — Antibody Blocker (40x) 1 x 100ml 1 x 50ml 2 x 15ml Blocker A 1 x 120ml 2 x 18ml 1 x 12ml Blocker B 1 x 120ml 2 x 18ml 1 x 12ml DAB Chromogen (20x) 1 x 6ml 1 x 2ml 1 x 1.5ml DAB Substrate 1 x 120ml 2 x 18ml 1 x 15ml Emerald Chromogen 1 x 120ml 2 x 18ml 1 x 15ml Mouse HRP Polymer 1 x 60ml 1 x 18ml 1 x 6ml Mouse Primer 1 x 120ml 1 x 36ml 1 x 12ml 4 x 9ml 1 x 12ml Permanent Red Activator (5x) 2 x 12ml 1 x 7.2ml 1 x 3ml Permanent Red Chromogen (100x) 1 x 1.2ml 1 x 360µl 1 x 150µl Permanent Red Substrate 1 x 120ml 2 x 18ml 1 x 15ml Plastic pipette 1 unit 1 unit 1 unit Rabbit AP Polymer 1 x 60ml 1 x 18ml 1 x 6ml Rabbit HRP Polymer 1 x 120ml 2 x 18ml 1 x 12ml
Our Abpromise guarantee covers the use of ab183295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183295 has not yet been referenced specifically in any publications.