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Kits/ Lysates/ Other Kits IHC tools Multicolor chromogenic
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TripleStain IHC Kit: R&R&M on rodent tissue (DAB, AP/Red & DAB/Ni) (ab183296)

  • Datasheet
  • SDS
  • Protocol Booklet
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Overview

  • Product name

    TripleStain IHC Kit: R&R&M on rodent tissue (DAB, AP/Red & DAB/Ni)
  • Assay type

    Polymer Multiplex
  • Product overview

    The TripleStain IHC Kit (ab183296) is designed to use with user supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell samples. The kit has been tested on tissue specimens that are paraffin embedded. For frozen tissue a lower temperature of 65ºC must be used for Antibody Blocker to prevent tissue from dissociating from slide. 

     

    Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-HRP anti-rabbit IgG, and polymer-AP anti-rabbit IgG with three substrates/chromogens; DAB (brown), DAB-Ni (black), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides users with the ability to permanently mount slides with a coverslip.

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 24 ml 72 ml 240 ml
    Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml
    Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml
    Blocker A 1 x 12ml 2 x 18ml 1 x 120ml
    Blocker B 1 x 12ml 2 x 18ml 1 x 120ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    DAB-Ni Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse Primer 1 x 12ml 1 x 36ml 1 x 120ml
    Nickel Solution (7x) 1 x 3ml 1 x 6ml 1 x 18ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rabbit HRP Polymer 1 x 12ml 2 x 18ml 1 x 120ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Multicolor chromogenic

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab183296 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab183296? Please let us know so that we can cite the reference in this datasheet.

    ab183296 has not yet been referenced specifically in any publications.

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