TripleStain IHC Kit: R&R&M on rodent tissue (DAB, AP/Red & DAB/Ni) (ab183296)

The TripleStain IHC Kit (ab183296) is designed to use with user supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell samples. The kit has been tested on tissue specimens that are paraffin embedded. For frozen tissue a lower temperature of 65ºC must be used for Antibody Blocker to prevent tissue from dissociating from slide. 

Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam’s TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-HRP anti-rabbit IgG, and polymer-AP anti-rabbit IgG with three substrates/chromogens; DAB (brown), DAB-Ni (black), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides users with the ability to permanently mount slides with a coverslip.

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