TripleStain IHC Kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green) (ab183295)

Overview

  • Product name
    TripleStain IHC Kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green)
  • Assay type
    Polymer Multiplex
  • Product overview

    Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested on tissue specimens that are paraffin embedded; however it may also be used on frozen or freshly prepared monolayer cell smears.

     

    Triple staining uses traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183295) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit HRP Polymer and Rabbit AP Polymer with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). Abcam's TripleStain IHC Kit (ab183295) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow the user to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with coverslip.

  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 240 ml 72 ml 24 ml
    Non-aqueous Mounting Medium
    Antibody Blocker (40x) 1 x 100ml 1 x 50ml 2 x 15ml
    Blocker A 1 x 120ml 2 x 18ml 1 x 12ml
    Blocker B 1 x 120ml 2 x 18ml 1 x 12ml
    DAB Chromogen (20x) 1 x 6ml 1 x 2ml 1 x 1.5ml
    DAB Substrate 1 x 120ml 2 x 18ml 1 x 15ml
    Emerald Chromogen 1 x 120ml 2 x 18ml 1 x 15ml
    Mouse HRP Polymer 1 x 60ml 1 x 18ml 1 x 6ml
    Mouse Primer 1 x 120ml 1 x 36ml 1 x 12ml
    2 x 18ml 1 x 12ml
    Permanent Red Activator (5x) 2 x 12ml 1 x 7.2ml 1 x 3ml
    Permanent Red Chromogen (100x) 1 x 1.2ml 1 x 360µl 1 x 150µl
    Permanent Red Substrate 1 x 120ml 2 x 18ml 1 x 15ml
    Rabbit AP Polymer 1 x 60ml 1 x 18ml 1 x 6ml
    Rabbit HRP Polymer 1 x 120ml 2 x 18ml 1 x 12ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Protocols

References

ab183295 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Here are answers to your questions.

For the TripleStain IHC kit I have some questions about the protocol attached
Q1. Can cells block with 3% H2O2 and levamisole
A1. Yes, 3% H2O2 and levamisole can be used to block endogenous HRP and A...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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