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    tris-buffered-saline-tbs-casein-elisa-reagent-ab171533.pdf

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Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)

  • Datasheet
  • SDS
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Overview

  • Product name

    Tris Buffered Saline (TBS) Casein ELISA Reagent
    See all ELISA buffer reagents
  • Tested applications

    Suitable for: ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • General notes

    Abcam’s Tris Buffered Saline (TBS) Casein ELISA Reagent consists of many phosphoserine groups that function to bind Ca2+. The 10X solution is ready for dilution to specific application requirements. ab171533 contributes to the minimization of non-specific interactions of the secondary reagents with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. Tris Buffered Saline (TBS), pH 7.6, is a poor buffer below pH 7.5. Tris has the disadvantages of its reactivity as a primary amine and its appreciable solubility in organic solvents, which can lead to an accumulation in the biological phase of a reaction system. To avoid complex formation with ionic species such as calcium and magnesium in blood, tris buffers are preferable over phosphate buffers.

    Storage Instructions:
    Store at room temperature. This product should be clear and colorless in appearance. To minimize the risk of contamination, a fresh working solution should be prepared each day or store unused working solutions at the recommended storage temperature. If proper technique is not performed the possibility of introducing contaminants is always possible.

    Product Use:
    ab171533 should be diluted 10X with reagent quality water. To produce a 1X working solution, dilute 1 mL of Tris Buffered Saline (TBS) Casein ELISA Reagent in 9 mL reagent quality water. Allow working solution to equilibrate to room temperature (25°C) prior to use. To use as a blocking reagent, treat target surface by covering completely with solution. To use working solution as a diluent, dilute antigen/antibody to desired concentration and treat surface by covering completely with solution. When solution is used for coating plates are obtained at +4°C.

Properties

  • Form

    Liquid
  • Storage instructions

    Please see notes section.
  • Storage buffer

    pH: 7
    Constituent: Casein
  • Concentration information loading...

Associated products

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)
    • AP Stability Conjugate Reagent (ab171536)
    • HRP Stability Conjugate Reagent (ab171537)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-Fr or IHC-P.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab171533? Please let us know so that we can cite the reference in this datasheet.

ab171533 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

What is the optimal pH range for diluted ab171533?

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Abcam community

Verified customer

Asked on May 14 2013

Answer

The optimal pH range for diluted TBSC is 7.4 - 7.8 at 22oC to 28oC.

Read More

Abcam Scientific Support

Answered on May 14 2013

Question

For how long should plates be incubated when using ab171533?

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Abcam community

Verified customer

Asked on May 14 2013

Answer

When using 1X solution for coating plates, best results are obtained following an overnight incubation at 2oC to 8oC.

Read More

Abcam Scientific Support

Answered on May 14 2013

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