Key features and details
- Rabbit polyclonal to Tristetraprolin/TTP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Tristetraprolin/TTP antibody
See all Tristetraprolin/TTP primary antibodies
DescriptionRabbit polyclonal to Tristetraprolin/TTP
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog
- HepG2 whole cell lysate (ab7900).
This product was previously labelled as Tristetraprolin
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab33058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 µg/ml.|
FunctionmRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
Sequence similaritiesContains 2 C3H1-type zinc fingers.
modificationsPhosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
Cellular localizationNucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
- Information by UniProt
- G0/G1 switch regulatory protein 24 antibody
- G0S24 antibody
- GOS24 antibody
ab33058 (4µg/ml) staining Tristetraprolin/TTP in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic and nuclei of macrophages and nuclei staining in some pneumocytes. There was also cytoplasmic and nuclei staining of epithelium cells of the bronchioles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal ampli
ICC/IF image of ab33058 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33058, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab33058 has been referenced in 10 publications.
- Wang H et al. Dysregulation of tristetraprolin and human antigen R promotes gastric cancer progressions partly by upregulation of the high-mobility group box 1. Sci Rep 8:7080 (2018). PubMed: 29728635
- Wu X et al. The ARE-binding protein Tristetraprolin (TTP) is a novel target and mediator of calcineurin tumor suppressing function in the skin. PLoS Genet 14:e1007366 (2018). PubMed: 29723192
- Guo J et al. Tristetraprolin Overexpression in Gastric Cancer Cells Suppresses PD-L1 Expression and Inhibits Tumor Progression by Enhancing Antitumor Immunity. Mol Cells 41:653-664 (2018). PubMed: 29936792
- Khalaj K et al. RNA binding protein, tristetraprolin in a murine model of recurrent pregnancy loss. Oncotarget 7:72486-72502 (2016). ICC/IF ; Mouse . PubMed: 27732963
- Deng K et al. Tristetraprolin inhibits gastric cancer progression through suppression of IL-33. Sci Rep 6:24505 (2016). WB . PubMed: 27074834
- Blanco FF et al. Transforming growth factor ß regulates P-body formation through induction of the mRNA decay factor tristetraprolin. Mol Cell Biol 34:180-95 (2014). PubMed: 24190969
- Chowdhury S et al. IL-17 Attenuates Degradation of ARE-mRNAs by Changing the Cooperation between AU-Binding Proteins and microRNA16. PLoS Genet 9:e1003747 (2013). PubMed: 24086143
- Shi JX et al. MK2 post-transcriptionally regulates TNF-a-induced expression of ICAM-1 and IL-8 via tristetraprolin in human pulmonary microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol : (2012). WB ; Human . PubMed: 22268119
- Ness GC et al. Involvement of tristetraprolin in transcriptional activation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase by insulin. Biochem Biophys Res Commun 420:178-82 (2012). WB ; Rat . PubMed: 22405826
- Masuda K et al. Tissue- and age-dependent expression of RNA-binding proteins that influence mRNA turnover and translation. Aging (Albany NY) 1:681-98 (2009). WB, ICC/IF ; Human . PubMed: 20157551