Anti-Tristetraprolin/TTP antibody (ab83579)
Key features and details
- Rabbit polyclonal to Tristetraprolin/TTP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tristetraprolin/TTP antibody
See all Tristetraprolin/TTP primary antibodies -
Description
Rabbit polyclonal to Tristetraprolin/TTP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Jurkat; Ramos; A431; SK N SH; PC3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).
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| Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). -
Sequence similarities
Contains 2 C3H1-type zinc fingers. -
Post-translational
modificationsPhosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation. -
Cellular localization
Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules. - Information by UniProt
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Database links
- Entrez Gene: 7538 Human
- Omim: 190700 Human
- SwissProt: P26651 Human
- Unigene: 534052 Human
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Alternative names
- G0/G1 switch regulatory protein 24 antibody
- G0S24 antibody
- GOS24 antibody
see all
Images
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Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)All lanes : Anti-Tristetraprolin/TTP antibody (ab83579) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutesTristetraprolin contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The expression profile observed is also consistent with what has been described in the literature (PMID:16508015).
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Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)This image is courtesy of an anonymous AbreviewAnti-Tristetraprolin/TTP antibody (ab83579) at 1/1000 dilution + HeLa whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab83579 has been referenced in 6 publications.
- Cao H & Sethumadhavan K Regulation of Cell Viability and Anti-inflammatory Tristetraprolin Family Gene Expression in Mouse Macrophages by Cottonseed Extracts. Sci Rep 10:775 (2020). PubMed: 31964945
- Sin-Chan P et al. A C19MC-LIN28A-MYCN Oncogenic Circuit Driven by Hijacked Super-enhancers Is a Distinct Therapeutic Vulnerability in ETMRs: A Lethal Brain Tumor. Cancer Cell 36:51-67.e7 (2019). PubMed: 31287992
- Li P et al. Tristetraprolin attenuates brain edema in a rat model of cerebral hemorrhage. Brain Behav 9:e01187 (2019). PubMed: 30729695
- Khalaj K et al. A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients. Sci Rep 7:5883 (2017). PubMed: 28724967
- Krishnamurthy PM et al. Involvement of p38-ßTrCP-Tristetraprolin-TNFa axis in radiation pneumonitis. Oncotarget 8:47767-47779 (2017). PubMed: 28548957
- Ray D et al. Tristetraprolin mediates radiation-induced TNF-a production in lung macrophages. PLoS One 8:e57290 (2013). PubMed: 23468959