Anti-Tristetraprolin/TTP antibody (ab83579)

Reviews (2)Q&A (1)References (5)
Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)
  • Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)

Key features and details

  • Rabbit polyclonal to Tristetraprolin/TTP
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Tristetraprolin/TTP antibody
    See all Tristetraprolin/TTP primary antibodies

  • Description

    Rabbit polyclonal to Tristetraprolin/TTP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Tristetraprolin/TTP aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P26651
    (Peptide available as ab106207)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Jurkat; Ramos; A431; SK N SH; PC3.

  • General notes

     This product was previously labelled as Tristetraprolin

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab83579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).

  • Sequence similarities

    Contains 2 C3H1-type zinc fingers.

  • Post-translational
    modifications

    Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.

  • Cellular localization

    Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt

  • Database links

    • Alternative names

      • G0/G1 switch regulatory protein 24 antibody
      • G0S24 antibody
      • GOS24 antibody
      • Growth factor-inducible nuclear protein NUP475 antibody
      • NUP475 antibody
      • Protein TIS11A antibody
      • RNF162A antibody
      • TIS 11 antibody
      • TIS11 antibody
      • TIS11A antibody
      • Tristetraprolin antibody
      • Tristetraproline antibody
      • TTP antibody
      • TTP_HUMAN antibody
      • Zfp-36 antibody
      • ZFP36 antibody
      • Zinc finger protein 36 antibody
      • Zinc finger protein 36 homolog antibody
      • Zinc finger protein 36, C3H type, homolog (mouse) antibody
      • Zinc finger protein, C3H type, 36 homolog antibody
      see all

    Images

    • Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)
      Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)
      All lanes : Anti-Tristetraprolin/TTP antibody (ab83579) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate
      Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 44 kDa why is the actual band size different from the predicted?
      Additional bands at: 150 kDa, 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 10 minutes


      Tristetraprolin contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The expression profile observed is also consistent with what has been described in the literature (PMID:16508015).

    • Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)
      Western blot - Anti-Tristetraprolin/TTP antibody (ab83579)This image is courtesy of an anonymous Abreview
      Anti-Tristetraprolin/TTP antibody (ab83579) at 1/1000 dilution + HeLa whole cell lysate at 20 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 44 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute

      See Abreview

    References (5)

    Publishing research using ab83579? Please let us know so that we can cite the reference in this datasheet.

    ab83579 has been referenced in 5 publications.

    • Cao H & Sethumadhavan K Regulation of Cell Viability and Anti-inflammatory Tristetraprolin Family Gene Expression in Mouse Macrophages by Cottonseed Extracts. Sci Rep 10:775 (2020). PubMed: 31964945
    • Li P  et al. Tristetraprolin attenuates brain edema in a rat model of cerebral hemorrhage. Brain Behav 9:e01187 (2019). PubMed: 30729695
    • Khalaj K  et al. A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients. Sci Rep 7:5883 (2017). PubMed: 28724967
    • Krishnamurthy PM  et al. Involvement of p38-ßTrCP-Tristetraprolin-TNFa axis in radiation pneumonitis. Oncotarget 8:47767-47779 (2017). PubMed: 28548957
    • Ray D  et al. Tristetraprolin mediates radiation-induced TNF-a production in lung macrophages. PLoS One 8:e57290 (2013). PubMed: 23468959

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Tristetraprolin/TTP antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Specification
    Hela
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tristetraprolin/TTP antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 15 2011

    Question

    I have asked for "sample size" antibody, if you have them available. The "100 ug" is a full-size vial which would suffice for at least a dozen of WB applications. We just would like to see how the Ab worked and if they would distinguish the isoforms (ZFP36, L1 and L2) before buying the full size.

    Read More
    Answer

    Thank you for your reply.

    I have heard back from the lab regarding ab83579:


    Crossreactivity with TISIIB and TISIID isn't something we have tested in house but an immunogen search shows that both TISIIB and TISIID only have 4 residues of the immunogen in common so we would say it is very unlikely that ab83579 would detect these 2 proteins.
    We do not offer smaller samples sizes than our selling size (due to operational resons), but have a guarantee in place that covers the antibody for 6 months after the purchase for the use in the tested species and applications as listed on the datasheet (https://www.abcam.com/Abpromise).
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

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