Key features and details
- Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP
- Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Tristetraprolin/TTP antibody [OTI8B5]
See all Tristetraprolin/TTP primary antibodies
DescriptionMouse monoclonal [OTI8B5] to Tristetraprolin/TTP
Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Tristetraprolin/TTP.
Database link: NP_003398
- HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA; Human colon, colon adenocarcinoma, ovary, endometrium and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY Tristetraprolin/TTP.
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 8B5 to OTI8B5, both clone numbers name the same antibody clone.
This product was previously labelled as Tristetraprolin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS
Our Abpromise guarantee covers the use of ab124024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1 - 1/1000.|
FunctionmRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
Sequence similaritiesContains 2 C3H1-type zinc fingers.
modificationsPhosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
Cellular localizationNucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
- Information by UniProt
- G0/G1 switch regulatory protein 24 antibody
- G0S24 antibody
- GOS24 antibody
All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA
Lysates/proteins at 5 µg per lane.
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202049)
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon tissue by Immunohistochemistry.
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human ovary tissue by Immunohistochemistry.
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human endometrium tissue by Immunohistochemistry.
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human prostate tissue by Immunohistochemistry.
ab124024, at 1/100, staining Tristetraprolin/TTP in COS7 cells transiently transfected with pCMV6-ENTRY Tristetraprolin/TTP by Immunofluorescence.
ab124024 at 1/100 dilution staining Tristetraprolin/TTP in HEK293T cells transfected with either pCMV6-ENTRY Tristetraprolin/TTP (Red) or empty vector control plasmid (Blue) by flow cytometry.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab124024 has been referenced in 1 publication.
- Selmi T et al. ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly. BMC Cancer 15:357 (2015). PubMed: 25939870