Recombinant Anti-TRK fused gene antibody [EPR8766] (ab156866)


  • Product name
    Anti-TRK fused gene antibody [EPR8766]
    See all TRK fused gene primary antibodies
  • Description
    Rabbit monoclonal [EPR8766] to TRK fused gene
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TRK fused gene. The exact sequence is proprietary.

  • Positive control
    • Human fetal brain, HeLa, A549, MCF-7 and 293T cell lysates; Human colon tissue and Human thyroid carcinoma tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab156866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
ICC/IF 1/1000.

For unpurified use at 1/100 - 1/250

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Tissue specificity
    • Involvement in disease
      Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ36137 antibody
      • HMSNP antibody
      • OTTHUMP00000214045 antibody
      • OTTHUMP00000214046 antibody
      • OTTHUMP00000214047 antibody
      • OTTHUMP00000214048 antibody
      • Protein TFG antibody
      • SPG57 antibody
      • TF6 antibody
      • TFG antibody
      • TFG_HUMAN antibody
      • TRK fused antibody
      • TRK fused gene antibody
      • TRK fused gene protein antibody
      • TRK-fused gene protein antibody
      • TRKT3 antibody
      • TRKT3 oncogene antibody
      see all


    • All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling TRK fused gene with purified ab156866 at 1/1000. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control. 

    • Immunoprecipitaion analysis in 293T cell lysate of TRK fused gene immunoprecipitated with ab156866 at 1/10.

      Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution + Immunoprecipitation pellets from 293T cell lysate at 10 µg
    • Immunofluorescent analysis of HeLa cells labeling TRF fused gene with ab156866 at 1/100.
    • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRF fused gene with ab156966 at 1/50.
    • Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling TRF fused gene with ab156866 at 1/50.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Yamamotoya T  et al. Trk-fused gene (TFG) regulates pancreatic ß cell mass and insulin secretory activity. Sci Rep 7:13026 (2017). Read more (PubMed: 29026155) »
    • Kanadome T  et al. The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein. FEBS J 284:56-76 (2017). Read more (PubMed: 27813252) »
    See all 2 Publications for this product

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