Recombinant Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TrkA + TrkB antibody [EPR1104(2)] -
Description
Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and SH SY5Y lysates; Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1104(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 87 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Cellular localization
TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. -
Database links
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 59109 Rat
- Omim: 191315 Human
- Omim: 600456 Human
see all
Images
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All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1.118 µg/ml (purified)
Lanes 1 & 3 & 5 : Empty vector overexpression 293T cell lysates
Lane 2 : His-Human TrkC overexpression 293T cell lysates
Lane 4 : His-Human TrkA overexpression 293T cell lysates
Lane 6 : His-Human TrkB overexpression 293T cell lysates
Lane 7 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 87 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
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ab109010 at 1/250 dilution staining TrkA + TrkB in Human brain by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDa -
ab109010 showing negative staining in Normal human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab109010 showing negative staining in Normal human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab109010 showing negative staining in Normal human tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab109010 showing negative staining in Normal human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109010 has been referenced in 1 publication.
- Peng T et al. Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression. Oxid Med Cell Longev 2022:8445093 (2022). PubMed: 36285300