Product nameAnti-TrkA + TrkB antibody [EPR1104(2)]
DescriptionRabbit monoclonal [EPR1104(2)] to TrkA + TrkB
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TrkA aa 750-850. The exact sequence is proprietary.
- Human fetal brain and SH SY5Y lysates; Human brain tissue.
A trial size is available to purchase for this antibody.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 87 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
Cellular localizationTrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane.
All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1.118 µg/ml (purified)
Lanes 1 & 3 & 5 : Empty vector overexpression 293T cell lysates
Lane 2 : His-Human TrkC overexpression 293T cell lysates
Lane 4 : His-Human TrkA overexpression 293T cell lysates
Lane 6 : His-Human TrkB overexpression 293T cell lysates
Lane 7 : Human brain lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 87 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
ab109010 at 1/250 dilution staining TrkA + TrkB in Human brain by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH SY5Y lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDa
ab109010 showing negative staining in Normal human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ab109010 showing negative staining in Normal human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ab109010 showing negative staining in Normal human tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ab109010 showing negative staining in Normal human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ab109010 has not yet been referenced specifically in any publications.