Product nameAnti-TrkA antibody
See all TrkA primary antibodies
DescriptionRabbit polyclonal to TrkA
Tested applicationsSuitable for: IP, WB, IHC (PFA fixed), IHC-FoFrmore details
Species reactivityReacts with: Rat
Extracellular fragment from the rat Trk A receptor (amino acids 1-416)
- rat dorsal root ganglion, rat brain tissue (eg. supra-optic nucleus)
General notesConcentration may vary depending on the batch. Current batch concentration is 3.8mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.81% Sodium chloride, 0.16% Sodium phosphate, 0.02% Potassium chloride, 0.04% Potassium phosphate
No preservative, sterile filtered
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab8871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. Use at a concentration of 2 - 5 µg/30µl Protein A sepharose per sample. Incubate for 2-3 hours at 4°C, then wash the Sepharose beads four times with lysis buffer. Elute sepharose-bound proteins in 2x Laemmli Sample buffer.|
|WB||Use a concentration of 1 µg/ml.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
FunctionReceptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
Tissue specificityIsoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
Involvement in diseaseCongenital insensitivity to pain with anhidrosis
Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein kinase domain.
DomainThe transmembrane domain mediates interaction with KIDINS220.
The extracellular domain mediates interaction with NGFR.
modificationsLigand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
Cellular localizationCell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
- Information by UniProt
- gp140trk antibody
- High affinity nerve growth factor receptor antibody
- High affinity nerve growth factor receptor precursor antibody
Immunofluorescent staining for TrkA obtained with TrkA antibody (ab8871) in rat DRG. Picture taken with X10 objective. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : IHC on 30um cryostat sections. Primary antibody ab8871 was used at 1/3000 and incubated overnight at room temperature. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2 hours at room temperature.
All lanes : Anti-TrkA antibody (ab8871) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from rat PC12 cells, un-treated.
Lane 2 : Whole cell lysate prepared from rat PC12 cells, treated with 10 ng/ml NGF for 5 minutes.
Lysates/proteins at 200000 cells per lane.
All lanes : HRP Goat anti-rabbit at 1/10000 dilution
Observed band size: 110,140 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Kucharczyk M et al. The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus. Psychoneuroendocrinology 94:121-133 (2018). WB ; Rat . Read more (PubMed: 29775875) »
- Hurst LA et al. TNFa drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun 8:14079 (2017). IF . Read more (PubMed: 28084316) »