• Product name
  • Description
    Rabbit polyclonal to TrkA
  • Host species
  • Tested applications
    Suitable for: IP, WB, IHC (PFA fixed), IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Extracellular fragment from the rat Trk A receptor (amino acids 1-416)

  • Positive control
    • rat dorsal root ganglion, rat brain tissue (eg. supra-optic nucleus)
  • General notes
    Concentration may vary depending on the batch. Current batch concentration is 3.8mg/ml.



Our Abpromise guarantee covers the use of ab8871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use at a concentration of 2 - 5 µg/30µl Protein A sepharose per sample. Incubate for 2-3 hours at 4°C, then wash the Sepharose beads four times with lysis buffer. Elute sepharose-bound proteins in 2x Laemmli Sample buffer.
WB Use a concentration of 1 µg/ml.
IHC (PFA fixed) Use at an assay dependent concentration.
IHC-FoFr 1/3000.


  • Function
    Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
    Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
  • Tissue specificity
    Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
  • Involvement in disease
    Congenital insensitivity to pain with anhidrosis
    Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • Domain
    The transmembrane domain mediates interaction with KIDINS220.
    The extracellular domain mediates interaction with NGFR.
  • Post-translational
    Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
    N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
    Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
  • Cellular localization
    Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • gp140trk antibody
    • High affinity nerve growth factor receptor antibody
    • High affinity nerve growth factor receptor precursor antibody
    • MTC antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • NTRK1 antibody
    • NTRK1_HUMAN antibody
    • Oncogene TRK antibody
    • p14-TrkA antibody
    • p140 TrkA antibody
    • p140-TrkA antibody
    • Slow nerve growth antibody
    • Trk A antibody
    • TRK antibody
    • Trk-A antibody
    • TRK1 antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • Tropomyosin-related kinase A antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    see all


  • Immunofluorescent staining for TrkA obtained with TrkA antibody (ab8871) in rat DRG. Picture taken with X10 objective. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT.  Protocol : IHC on 30um cryostat sections. Primary antibody ab8871 was used at 1/3000 and incubated overnight at room temperature. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2 hours at room temperature.
  • All lanes : Anti-TrkA antibody (ab8871) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from rat PC12 cells, un-treated.
    Lane 2 : Whole cell lysate prepared from rat PC12 cells, treated with 10 ng/ml NGF for 5 minutes.

    Lysates/proteins at 200000 cells per lane.

    All lanes : HRP Goat anti-rabbit at 1/10000 dilution

    Observed band size: 110,140 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:
  • Kucharczyk M  et al. The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus. Psychoneuroendocrinology 94:121-133 (2018). WB ; Rat . Read more (PubMed: 29775875) »
  • Hurst LA  et al. TNFa drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun 8:14079 (2017). IF . Read more (PubMed: 28084316) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Mouse Cultured Cells (dorsal root ganglion)
dorsal root ganglion
Yes - cold methanol for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Dr. Zunyi Wang

Verified customer

Submitted Feb 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (PC12)
Loading amount
200000 cells
Lane 1 Untreated Lane 2: NGF 10 ng/ml, 5 minutes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Joe Makkerh

Verified customer

Submitted Oct 01 2007


Thank you for your email and I'm very pleased to hear that the antibody worked well for you. We cannot provide you with any complimentary vials, but I do suggest that you submit an Abreview and in return we'll award you points that can be used in exchange for discounts off of future purchases or for other various rewards. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. We hope to receive your Abreview very shortly. Thank you for your time and effort.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Rat Tissue sections (Rat brain, spinal cord, dorsal root ganglion)
Rat brain, spinal cord, dorsal root ganglion

Dr. Sophie Pezet

Verified customer

Submitted Apr 12 2006


Thank you for your enquiry. The antibody comes in liquid form. It would depend on the lot ordered, but the last lot we shipped was 100 micrograms (3.8 mg/ml). The antibody is shipped in PBS (0.14 M Sodium Chloride; 0.003 M Potassium Chloride; 0.002 M Potassium Phosphate; 0.01 M Sodium Phosphate; pH 7.4), no preservative. Please do not hesitate to contact us if you need further information,

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The concentration is 2.0 mg/ml

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The concentration will vary from lot to lot. The concentration of the recent lot we currently sell is 2.0 mg/ml. Regarding IHC on paraffin sections, originally this antibody has not been tested for this application. However, one of our customers reported that this antibody works nicely. Please take a look at the published review submitted by J. Timmermans. If you need anything further, please do not hesitate to contact us again.

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We have had no feedback on cross-reactivity with human and have not seen any published data that would confirm or deny it.

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Thank you for your enquiry. All the information that we have regarding ab8871 is located on the online datasheet. I am sorry but we do not have an example of a Western blot for this antibody.

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Many thanks for sending me your detailed protocol. I have taken a look at it and I have a few suggestions: 1) Only fix the specimins for 10 minutes in Zamboni's fixative. 2 hours may be too long, as the paraformaldehyde could cross-link the proteins to an extent where the epitope is masked. 2) H2O2 can negatively effect epitopes, since it is quite harsh. I have personally found that endogenous peroxidases are adequately blocked after only 5 minutes exposure to 3% H2O2. 3) Please try the secondary antibody at 1:250 and the tertiary HRP-ABC at 1:100. Even though laboratories have a standard protocol, a higher degree of signal amplification is required for some antibodies, especially if the antigen is present in low concentrations. We have no data for the cross-reactivity of this antibody with mouse. I suggest that you try it on rat, as we have shown cross-reactivity with this species.

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