Recombinant
RabMAb

Recombinant Anti-TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)

Overview

  • Product name

    Anti-TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140]
    See all TrkA+TrkB+TrkC primary antibodies
  • Description

    Rabbit monoclonal [EPR19140] to TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TrkB aa 500-600 (phospho Y516). The exact sequence is proprietary.
    Database link: Q16620

  • Positive control

    • WB: HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag; HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes; PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 92 kDa).

Target

Images

  • All lanes : Anti-TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution

    Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/10000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y516A mutant) with Myc-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).

  • All lanes : Anti-TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).

  • Dot blot analysis of TrkA (phospho Y490), TrkB (phospho Y516), and TrkC (phospho Y516) peptides labeled with ab197071 at 1/1000 dilution.

    Lane 1: TrkB (phospho Y516) phospho peptide;

    Lane 2: TrkB Non-phospho peptide;

    Lane 3: TrkA (phospho Y490) phospho peptide;

    Lane 4: TrkA Non-phospho peptide;

    Lane 5: TrkC (phospho Y516) phospho peptide;

    Lane6: TrkC Non-phospho peptide.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Note: ab197071 reacts with TrkB (phospho Y516)/ TrkA (phospho Y490)/ TrkC (phospho Y516) according to the dot blot data.

References

ab197071 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197071.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up